desulfurobacterium_crinifex_medium

Overview

External ontology

mediadive.medium:829c (source link unavailable) — DESULFUROBACTERIUM CRINIFEX MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 6.0, 'max': 6.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829c.pdf The original reports final volume 1011 ml, while its listed main liquid inputs sum to 1011.5 ml. Preserve the discrepancy. Source-reported basal rates do not certify independently measured final volume; no missing makeup water is invented. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.

Composition

flowchart LR
    CultureMech_001981["desulfurobacterium_crinifex_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_001981 --> ing0_Sea_Salt
    ing1_NH4Cl["NH4Cl"]
    CultureMech_001981 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KH2PO4["KH2PO4"]
    CultureMech_001981 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
    CultureMech_001981 --> ing3_MES__2__N_morpholino__ethane_sulfonic_acid_
    CHEBI_39005["CHEBI:39005"]
    ing3_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
    ing4_Sulfur["Sulfur"]
    CultureMech_001981 --> ing4_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing4_Sulfur -.-> CHEBI_26833
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001981 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001981 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_Distilled_water["Distilled water"]
    CultureMech_001981 --> ing7_Distilled_water

Ingredients (8)

Preparation

1. Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. Autoclave at a temperature of <b>110°C</b> for 20 min! Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 6.0 - 6.5, if necessary.
2. After inoculation use 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  DSM 16304 pH prescription

  Relationship

  STRAIN_SPECIFIC_VARIANT

  Modifications

  • Adjust pH to 5.5.

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829c.pdf

    Supports

    SUPPORT

    Snippet

    For DSM 16304: Adjust pH to 5.5.

    Explanation

    variants[0].name: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T04:03:54.547034+00:00; capture SHA256=a813a88f952417a5986d9f08de329db207f14bbb43b490f4c5d9821757fc2770. Additional exact excerpt: For DSM 16304: Adjust pH to 5.5. variants[0].relationship: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T04:03:54.547034+00:00; capture SHA256=a813a88f952417a5986d9f08de329db207f14bbb43b490f4c5d9821757fc2770. Additional exact excerpt: For DSM 16304: Adjust pH to 5.5. variants[0].modifications: Original strain-specific instruction, separate from the base recipe; no measured growth association is inferred. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-09T04:03:54.547034+00:00; capture SHA256=a813a88f952417a5986d9f08de329db207f14bbb43b490f4c5d9821757fc2770.

Provenance