Overview
- External ontology
- mediadive.medium:829d (source link unavailable) — DESULFUROBACTERIUM INDICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.0, 'max': 6.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium829d.pdf The original reports final volume 1020 ml, while its listed main liquid inputs sum to 1011.5 ml. Preserve the discrepancy. Source-reported basal rates do not certify independently measured final volume; no missing makeup water is invented. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.
Composition
flowchart LR
CultureMech_001982["desulfurobacterium_indicum_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_001982 --> ing0_Sea_Salt
ing1_NH4Cl["NH4Cl"]
CultureMech_001982 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KH2PO4["KH2PO4"]
CultureMech_001982 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MES__2__N_morpholino__ethane_sulfonic_acid_["MES [2-(N-morpholino) ethane sulfonic acid]"]
CultureMech_001982 --> ing3_MES__2__N_morpholino__ethane_sulfonic_acid_
CHEBI_39005["CHEBI:39005"]
ing3_MES__2__N_morpholino__ethane_sulfonic_acid_ -.-> CHEBI_39005
ing4_Na_acetate["Na-acetate"]
CultureMech_001982 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Sulfur["Sulfur"]
CultureMech_001982 --> ing5_Sulfur
CHEBI_26833["CHEBI:26833"]
ing5_Sulfur -.-> CHEBI_26833
ing6_Na2CO3["Na2CO3"]
CultureMech_001982 --> ing6_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing6_Na2CO3 -.-> CHEBI_29377
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_001982 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Distilled_water["Distilled water"]
CultureMech_001982 --> ing8_Distilled_water
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 29.4118 G_PER_L | Direct basal input 30 g / source-reported final volume 1.02 L = 29.4118 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. Literal source attribute: SIGMA, must not show signs of caking. | |
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.294118 G_PER_L |
CHEBI:31206
ammonium chloride
|
Direct basal input 0.3 g / source-reported final volume 1.02 L = 0.294118 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.343137 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Direct basal input 0.35 g / source-reported final volume 1.02 L = 0.343137 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 4 |
MES [2-(N-morpholino) ethane sulfonic acid]
C6H13NO4S 195.24 g/mol
|
1.91176 G_PER_L |
CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
|
Direct basal input 1.95 g / source-reported final volume 1.02 L = 1.91176 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.156863 G_PER_L |
CHEBI:32954
sodium acetate
|
Direct basal input 0.16 g / source-reported final volume 1.02 L = 0.156863 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 6 |
Sulfur
S 32.067 g/mol
|
9.80392 G_PER_L |
CHEBI:26833
sulfur atom
|
(powdered) Direct basal input 10 g / source-reported final volume 1.02 L = 9.80392 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. Literal source condition: powdered. |
| 7 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.490196 G_PER_L |
CHEBI:29377
sodium carbonate
|
Direct basal input 0.5 g / source-reported final volume 1.02 L = 0.490196 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.490196 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Direct basal input 0.5 g / source-reported final volume 1.02 L = 0.490196 g/L, six significant digits. This is the printed denominator, not independently measured volume; the liquid-input discrepancy is retained at recipe root. |
| 9 | Distilled water | 1 L | Preparation input 1000 ml / 1000 = 1 L, not mass, volume percent or measured final volume. |
Preparation
- Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. Autoclave at a temperature of <b>110°C</b> for 20 min! Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 6.0 - 6.5, if necessary.
- After inoculation add 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture.
Solutions
Modified Wolin's mineral solution
Dose: 0.01 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Nitrilotriacetic acid | Source batch input: 1.5 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| MgSO4 x 7 H2O | Source batch input: 3 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| MnSO4 x H2O | Source batch input: 0.5 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| NaCl | Source batch input: 1 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| FeSO4 x 7 H2O | Source batch input: 0.1 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| CoSO4 x 7 H2O | Source batch input: 0.18 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| CaCl2 x 2 H2O | Source batch input: 0.1 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| ZnSO4 x 7 H2O | Source batch input: 0.18 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| CuSO4 x 5 H2O | Source batch input: 0.01 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| AlK(SO4)2 x 12 H2O | Source batch input: 0.02 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| H3BO3 | Source batch input: 0.01 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Na2MoO4 x 2 H2O | Source batch input: 0.01 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| NiCl2 x 6 H2O | Source batch input: 0.03 g. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. Copied annotations do not establish this exact salt, hydrate or stereochemical form; archive them without inventing replacement identifiers. | |
| Na2SeO3 x 5 H2O | Source batch input: 0.3 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Na2WO4 x 2 H2O | Source batch input: 0.4 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Distilled water | 1 L | Preparation input 1000 ml / 1000 = 1 L, not mass, volume percent or measured final volume. |
| KOH | Unquantified mineral-stock titration: first NTA to pH 6.5, then completed stock to pH 7.0. These are not receiving-medium pH values or a fixed KOH dose. |
Receiving-batch addition 10 ml / 1000 = 0.01 L of prepared stock, not g/L or an inferred ml/L rate. Stock strength and preparation remain separate.
Sodium resazurin (0.1% w/v)
Dose: 0.0005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium resazurin | 0.1 PERCENT_W_V | Explicit 0.1% w/v indicator stock, not the concentration in receiving medium. Keep its separate 0.50 ml dose. Copied annotations do not establish this exact salt, hydrate or stereochemical form; archive them without inventing replacement identifiers. |
Receiving-batch addition 0.5 ml / 1000 = 0.0005 L of prepared stock, not g/L or an inferred ml/L rate. Stock strength and preparation remain separate.
Wolin's vitamin solution (10x)
Dose: 0.001 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | Source batch input: 20 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Folic acid | Source batch input: 20 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Pyridoxine hydrochloride | Source batch input: 100 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Thiamine HCl | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Riboflavin | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Nicotinic acid | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Calcium D-(+)-pantothenate | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. Copied annotations do not establish this exact salt, hydrate or stereochemical form; archive them without inventing replacement identifiers. | |
| Vitamin B12 | Source batch input: 1 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| p-Aminobenzoic acid | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| (DL)-alpha-Lipoic acid | Source batch input: 50 mg. No explicit final-volume/makeup statement was found for this stage in the inspected original. Retain the mass in notes; API volume/g_l and water input are not an independently verified denominator. | |
| Distilled water | 1 L | Preparation input 1000 ml / 1000 = 1 L, not mass, volume percent or measured final volume. |
Receiving-batch addition 1 ml / 1000 = 0.001 L of prepared stock, not g/L or an inferred ml/L rate. Stock strength and preparation remain separate.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.418252Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 829d |
| 2026-01-28T19:48:30.499084Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:40.351327Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:09.099414Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001982 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.615087+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFUROBACTERIUM INDICUM MEDIUM" to "desulfurobacterium_indicum_medium" for consistent matching |
| 2026-05-20T06:01:15.247756+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6-6.5' -> {'min': 6.0, 'max': 6.5} |
| 2026-06-05T07:40:58.305349+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.228031+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T04:52:49.323535+00:00 | codex | RESTORED_MEDIUM_829_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore exact-source medium-829 formulations and stock boundaries, preserve provider rates/nulls and source conflicts, repair quantity units and family scope, and retain prior evidence/history. Each scientific change is linked to an inspected source excerpt; no growth association inferred. |