reactivation_with_liquid_medium_830 (CultureMech:001987)

Overview

External ontology

mediadive.medium:830c (source link unavailable) — REACTIVATION WITH LIQUID MEDIUM 830

Applications

Microbial cultivation

pH

7.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830c.pdf

Composition

flowchart LR
    CultureMech_001987["reactivation_with_liquid_medium_830"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001987 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_001987 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_001987 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_001987 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_001987 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_001987 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001987 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001987 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_001987 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

#	Ingredient	Concentration	Ontology
1	Yeast extract	0.5 G_PER_L
2	Proteose peptone	0.5 G_PER_L
3	Casamino acids	0.5 G_PER_L
4	Glucose C6H12O6 180.156 g/mol	0.5 G_PER_L	CHEBI:17234 glucose
5	Starch	0.5 G_PER_L	CHEBI:28017 starch
6	Na-pyruvate C3H3O3.Na 110.044 g/mol	0.3 G_PER_L	CHEBI:50144 sodium pyruvate
7	K2HPO4 HO4P.2K 174.174 g/mol	0.3 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
8	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.05 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
9	Agar	15 G_PER_L	CHEBI:2509 agar

Preparation

Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:12.429041Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 830c
2026-01-28T19:48:34.547558Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:09.129354Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001987
2026-03-17T06:30:38.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.649724+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "REACTIVATION WITH LIQUID MEDIUM 830" to "reactivation_with_liquid_medium_830" for consistent matching
2026-06-05T07:41:44.112171+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:50.166539+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:10.881618+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.