r2a_medium_microaerobic

Overview

External ontology

mediadive.medium:830d (source link unavailable) — R2A MEDIUM (MICROAEROBIC)

Applications

• Microbial cultivation

pH range

{'min': 5.0, 'max': 5.5}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830d.pdf

Composition

flowchart LR
    CultureMech_001988["r2a_medium_microaerobic"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_001988 --> ing0_Yeast_extract
    ing1_Proteose_peptone["Proteose peptone"]
    CultureMech_001988 --> ing1_Proteose_peptone
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_001988 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_001988 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Starch["Starch"]
    CultureMech_001988 --> ing4_Starch
    CHEBI_28017["CHEBI:28017"]
    ing4_Starch -.-> CHEBI_28017
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_001988 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001988 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001988 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795

Ingredients (8)

Preparation

1. Dissolve ingredients, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g., Balch-type tubes or serum vials) to 30% volume (e.g., 5 ml medium in a 16 ml Hungate-type tube) under the 80% N2 and 20% CO2 gas atmosphere. Close vials with butyl rubber septa to prevent the free exchange of oxygen with the external atmosphere and autoclave them. Adjust pH of the autoclaved medium to 5.0 - 5.5 using a sterile stock solution of sodium carbonate (5% w/v), prepared under 80% N2 and 20% CO2 gas mixture.
2. Before inoculation, add sterile air to obtain 5% O2 in headspace gas atmosphere (e.g., add 2 ml air to a Hungate-type tube).

Target organisms

No target organisms are recorded.

Provenance