serpulina_murdochii_medium

CultureMech:001996 bacterial COMPLEX LIQUID
Original name: SERPULINA MURDOCHII MEDIUM

Overview

External ontology
mediadive.medium:840 (source link unavailable) — SERPULINA MURDOCHII MEDIUM
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium840.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_001996["serpulina_murdochii_medium"]
    ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_001996 --> ing0_Pancreatic_digest_of_casein
    ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_001996 --> ing1_Peptic_digest_of_soybean_meal
    ing2_Glucose["Glucose"]
    CultureMech_001996 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sodium_chloride["Sodium chloride"]
    CultureMech_001996 --> ing3_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing3_Sodium_chloride -.-> CHEBI_26710
    ing4_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_001996 --> ing4_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing4_Dipotassium_phosphate -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_001996 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_NaHCO3["NaHCO3"]
    CultureMech_001996 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Bovine_serum["Bovine serum"]
    CultureMech_001996 --> ing7_Bovine_serum
    ing8_Glucose["Glucose"]
    CultureMech_001996 --> ing8_Glucose
    ing8_Glucose -.-> CHEBI_17234
    ing9_Air["Air"]
    CultureMech_001996 --> ing9_Air

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
2 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
3 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
4 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 NaHCO3
CHO3.Na 84.006 g/mol
0.09 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 [Merged 2 duplicates: 0.03, 0.06]
8 Bovine serum 1.0499999999999998 G_PER_L [Merged 2 duplicates: 0.35, 0.7]
9 Glucose
C6H12O6 180.156 g/mol
0.045 G_PER_L CHEBI:17234
glucose
 [Merged 2 duplicates: 0.015, 0.03]
10 Air 5.5 G_PER_L [Merged 2 duplicates: 0.5, 5.0]

Preparation

  1. Incubate inoculated tubes in slanted position.
  2. Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in Hungate tubes (5 ml per tube).
  3. After autoclaving and cooling add (per tube):
  4. Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in 100 ml serum bottles (10 ml per bottle).
  5. After autoclaving and cooling add per bottle:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.460838Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 840
2026-03-13T03:08:45.515544Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:09.225842Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001996
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.758147+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SERPULINA MURDOCHII MEDIUM" to "serpulina_murdochii_medium" for consistent matching
2026-06-05T07:41:45.761662+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.