msv_thiotrix_medium

CultureMech:001997 bacterial DEFINED SOLID_AGAR
Original name: MSV-THIOTRIX-MEDIUM

Overview

External ontology
mediadive.medium:841 (source link unavailable) — MSV-THIOTRIX-MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium841.pdf

Composition

flowchart LR
    CultureMech_001997["msv_thiotrix_medium"]
    ing0_Agar["Agar"]
    CultureMech_001997 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001997 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001997 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001997 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001997 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_KH2PO4["KH2PO4"]
    CultureMech_001997 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_001997 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_001997 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_Na_acetate["Na-acetate"]
    CultureMech_001997 --> ing8_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing8_Na_acetate -.-> CHEBI_32954
    ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_001997 --> ing9_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001997 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Inositol["Inositol"]
    CultureMech_001997 --> ing11_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing11_Inositol -.-> CHEBI_24848
    ing12_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001997 --> ing12_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing12_Calcium_pantothenate -.-> CHEBI_31345
    ing13_Biotin["Biotin"]
    CultureMech_001997 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_001997 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_001997 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Agar 15 G_PER_L CHEBI:2509
agar
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.504541 G_PER_L CHEBI:62946
ammonium sulfate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.100908 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0504541 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 K2HPO4
HO4P.2K 174.174 g/mol
0.110999 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.0857719 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.00201816 G_PER_L CHEBI:86254
iron trichloride hexahydrate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.00302725 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 Na-acetate
C2H3O2.Na 82.034 g/mol
0.550959 G_PER_L CHEBI:32954
sodium acetate
10 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.403633 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
11 NaHCO3
CHO3.Na 84.006 g/mol
52.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Inositol
C6H12O6 180.156 g/mol
100 G_PER_L CHEBI:24848
inositol
13 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
14 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
15 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
16 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Final pH of appr. 7.4 should be controlled sterile.
  2. Autoclave for 20 min. at 121°C.
  3. NaHCO3 2.625 g per 50 ml distilled water
  4. Autoclave for 20 min. at 121°C and add 8 ml/l sterile solution II shortly, before inoculation of bacterial culture.
  5. H3P-vitamine solution (see medium 428)
  6. Filter sterilize vitamine solution and add 1 ml/l medium.
  7. 10% inositol (1g/10 ml distilled water)
  8. Filter sterilize solution IV and add 0.1 ml/l medium.
  9. Final pH of appr. 7.4 should be controlled sterile.
  10. Filter sterilize (0.2 µm pore-size) and store at 4°C.

Solutions

Standard vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Riboflavin 0.1 G_PER_L
Thiamine-HCl x 2 H2O 0.5 G_PER_L
Nicotinic acid 0.5 G_PER_L
Pyridoxine hydrochloride 0.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.462806Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 841
2026-03-13T06:19:09.235046Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001997
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.767530+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MSV-THIOTRIX-MEDIUM" to "msv_thiotrix_medium" for consistent matching
2026-06-05T07:41:38.829696+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.270559+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.705176+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Standard vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).