haloanaerobacter_salinarius_medium

CultureMech:001998 bacterial COMPLEX LIQUID
Original name: HALOANAEROBACTER SALINARIUS MEDIUM

Overview

External ontology
mediadive.medium:842 (source link unavailable) — HALOANAEROBACTER SALINARIUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium842.pdf

Composition

flowchart LR
    CultureMech_001998["haloanaerobacter_salinarius_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001998 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001998 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_001998 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NH4Cl["NH4Cl"]
    CultureMech_001998 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_KH2PO4["KH2PO4"]
    CultureMech_001998 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001998 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001998 --> ing6_Yeast_extract
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001998 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_NaHCO3["NaHCO3"]
    CultureMech_001998 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Glucose["Glucose"]
    CultureMech_001998 --> ing9_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing9_Glucose -.-> CHEBI_17234
    ing10_Resazurin["Resazurin"]
    CultureMech_001998 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001998 --> ing11_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing11_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001998 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_NaOH["NaOH"]
    CultureMech_001998 --> ing13_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing13_NaOH -.-> CHEBI_32145
    ing14_Biotin["Biotin"]
    CultureMech_001998 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001998 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_001998 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_Thiamine["Thiamine"]
    CultureMech_001998 --> ing17_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing17_Thiamine -.-> CHEBI_18385
    ing18_Na2_EDTA["Na2-EDTA"]
    CultureMech_001998 --> ing18_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing18_Na2_EDTA -.-> CHEBI_64734
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001998 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001998 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_001998 --> ing21_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing21_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing22_ZnCl2["ZnCl2"]
    CultureMech_001998 --> ing22_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing22_ZnCl2 -.-> CHEBI_49976
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001998 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001998 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_H3BO3["H3BO3"]
    CultureMech_001998 --> ing25_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing25_H3BO3 -.-> CHEBI_33118
    ing26_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001998 --> ing26_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing26_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
150 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
15 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
3 G_PER_L CHEBI:32588
potassium chloride
4 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
5 KH2PO4
H2O4P.K 136.084 g/mol
0.35 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Yeast extract 0.1 G_PER_L
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 Glucose
C6H12O6 180.156 g/mol
1.2 G_PER_L CHEBI:17234
glucose
11 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
12 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
13 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
14 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
15 Biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
18 Thiamine
C12H17N4OS 265.362 g/mol
0.1 G_PER_L CHEBI:18385
thiamine(1+)
19 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
20 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.05 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
23 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
27 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Final pH 7.2 - 7.4
  2. Prepare the medium without yeast extract, Na2S x 9 H2O, NaHCO3, glucose and vitamin solution (prepared as anaerobic, sterile stock solutions). Boil the medium and cool under anaerobic gas mixture of N2:CO2 (80:20). Dispense medium into Hungate tubes or serum bottles using the same gas mixture. Autoclave. Add the remaining components to the medium once it has cooled and check the final pH.
  3. Adjust pH to 6.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.466264Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 842
2026-03-13T06:19:09.247041Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001998
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:21.780758+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOANAEROBACTER SALINARIUS MEDIUM" to "haloanaerobacter_salinarius_medium" for consistent matching
2026-05-20T06:01:21.785132+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:41:07.378602+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.394046+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.