spirillum_winogradskyi_medium

Overview

External ontology

mediadive.medium:845 (source link unavailable) — SPIRILLUM WINOGRADSKYI MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.2, 'max': 7.4}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium845.pdf

Composition

flowchart LR
    CultureMech_002001["spirillum_winogradskyi_medium"]
    ing0_Peptone["Peptone"]
    CultureMech_002001 --> ing0_Peptone
    ing1_Disodium_succinate["Disodium succinate"]
    CultureMech_002001 --> ing1_Disodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing1_Disodium_succinate -.-> CHEBI_63675
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002001 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002001 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002001 --> ing4_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing5_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002001 --> ing5_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_x_H2O -.-> CHEBI_86364
    ing6_Agar["Agar"]
    CultureMech_002001 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Catalase["Catalase"]
    CultureMech_002001 --> ing7_Catalase
    ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_002001 --> ing8_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing9_ZnCl2["ZnCl2"]
    CultureMech_002001 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002001 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_002001 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002001 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002001 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002001 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002001 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002001 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17_D_____biotin["D-(+)-biotin"]
    CultureMech_002001 --> ing17_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_D_____biotin -.-> CHEBI_15956
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002001 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (19)

Preparation

1. Dissolve ingredients except trace elements, vitamins and catalase, adjust pH to 7.2 - 7.4, bring medium to the boil and cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. To complete the medium add trace elements, vitamins and catalase from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins and catalase should be sterilized by filtration. After inoculation, inject sterile air into the culture vessel up to about 20% of the total gas volume (i.e., 4 parts N2 and 1 part air).
2. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Solutions

Target organisms

No target organisms are recorded.

Provenance