rae_medium

Overview

External ontology

mediadive.medium:848 (source link unavailable) — RAE MEDIUM

Applications

• Microbial cultivation

pH

3.8

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium848.pdf

Composition

flowchart LR
    CultureMech_002005["rae_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_002005 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Peptone["Peptone"]
    CultureMech_002005 --> ing1_Peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_002005 --> ing2_Yeast_extract
    ing3_Citric_acid_x_H2O["Citric acid x H2O"]
    CultureMech_002005 --> ing3_Citric_acid_x_H2O
    CHEBI_31404["CHEBI:31404"]
    ing3_Citric_acid_x_H2O -.-> CHEBI_31404
    ing4_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002005 --> ing4_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing4_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing5_Glacial_acetic_acid["Glacial acetic acid"]
    CultureMech_002005 --> ing5_Glacial_acetic_acid
    ing6_Absolute_ethanol["Absolute ethanol"]
    CultureMech_002005 --> ing6_Absolute_ethanol
    ing7_Acetic_acid["Acetic acid"]
    CultureMech_002005 --> ing7_Acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing7_Acetic_acid -.-> CHEBI_15366
    ing8_Ethanol["Ethanol"]
    CultureMech_002005 --> ing8_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing8_Ethanol -.-> CHEBI_16236

Ingredients (9)

Preparation

1. Final pH ca. 3.8 (pH is not adjusted)
2. Both liquid and solid media are prepared by autoclaving the medium without the addition of glacial acetic acid and absolute ethanol. Glacial acetic acid and absolute ethanol are sterilised either by filtration (use Teflon filters) or they may be autoclaved in completely closed screw cap glass bottles sealed with a Teflon coated septum. The preparation of solid media employs a double layer system similar to that described for Acetobacter europaeus medium (see medium 631). Substitute the components listed above and prepare a lower, 0.5% agar layer, which is then overlaid with a 1.0% agar layer as described in medium 631. Incubate in closed containers to keep the humidity high (> 90%).
3. The ethanol and acetic acid are sterilized separately by filtration (use filter resistant to these solvents), and added aseptically to the sterile medium. Acetobacter europaeus will not grow on plates containing 1-2% agar, and a double layer system has to be used. A layer of growth medium containing 0.5% agar (about 35 ml) is poured into a plate and allowed to solidify. A thin layer (5-10 ml) of the growth medium containing 1% agar is then poured on top. Carefully streak the plates, which are best incubated in closed containers (e.g. plastic boxes) at 30°C.

Target organisms

No target organisms are recorded.

Provenance