Overview
- External ontology
- mediadive.medium:849 (source link unavailable) — AE MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium849.pdf
Composition
flowchart LR
CultureMech_002006["ae_medium"]
ing0_Glucose["Glucose"]
CultureMech_002006 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_002006 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_002006 --> ing2_Peptone
ing3_Absolute_ethanol["Absolute ethanol"]
CultureMech_002006 --> ing3_Absolute_ethanol
CHEBI_16236["CHEBI:16236"]
ing3_Absolute_ethanol -.-> CHEBI_16236
ing4_Glacial_acetic_acid["Glacial acetic acid"]
CultureMech_002006 --> ing4_Glacial_acetic_acid
CHEBI_15366["CHEBI:15366"]
ing4_Glacial_acetic_acid -.-> CHEBI_15366
ing5_Agar["Agar"]
CultureMech_002006 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Distilled_water["Distilled water"]
CultureMech_002006 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5.0 G_PER_L |
CHEBI:17234
glucose
|
DSMZ Medium 849 lists 5.0 g glucose. |
| 2 | Yeast extract | 3.0 G_PER_L |
FOODON:03315426
yeast extract
|
DSMZ Medium 849 lists 3.0 g yeast extract. |
| 3 | Peptone | 4.0 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
DSMZ Medium 849 lists 4.0 g peptone. |
| 4 |
Absolute ethanol
C2H6O 46.069 g/mol
|
30.0 ML_PER_L |
CHEBI:16236
ethanol
|
DSMZ Medium 849 lists 30.0 ml absolute ethanol. |
| 5 |
Glacial acetic acid
C2H4O2 60.052 g/mol
|
30.0 ML_PER_L |
CHEBI:15366
acetic acid
|
DSMZ Medium 849 lists 30.0 ml glacial acetic acid. |
| 6 | Agar | 9.0 G_PER_L |
CHEBI:2509
agar
|
DSMZ Medium 849 lists 9.0 g agar. |
| 7 |
Distilled water
H2O 18.015 g/mol
|
940.0 ML_PER_L |
CHEBI:15377
water
|
DSMZ Medium 849 lists 940.0 ml distilled water. |
Preparation
- Autoclave the medium without the addition of glacial acetic acid and absolute ethanol.
- Sterilize glacial acetic acid and absolute ethanol by filtration through Teflon filters or by autoclaving in completely closed screw-cap glass bottles sealed with Teflon-coated septa.
- Add sterile glacial acetic acid and absolute ethanol.
- Incubate plates in sealed containers to keep humidity high above 90%.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.497474Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 849 |
| 2026-03-13T06:19:09.336764Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002006 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:21.883022+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AE MEDIUM" to "ae_medium" for consistent matching |
| 2026-06-05T07:40:47.505271+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_dsmz_849_ae_score15.py | RESOLVED_DSMZ_849_AE_SCORE15 | DSMZ Medium 849 lists AE medium with glucose, yeast extract, peptone, 30.0 ml absolute ethanol, 30.0 ml glacial acetic acid, agar, and 940.0 ml distilled water. |