Overview
- External ontology
- mediadive.medium:859 (source link unavailable) — LACTOBACILLUS MEDIUM IV
- Applications
-
- Microbial cultivation
- pH
- 6.3
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium859.pdf
Composition
flowchart LR
CultureMech_002017["lactobacillus_medium_iv"]
ing0_Tryptone["Tryptone"]
CultureMech_002017 --> ing0_Tryptone
ing1_Meat_extract["Meat extract"]
CultureMech_002017 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_002017 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_002017 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Maltose["Maltose"]
CultureMech_002017 --> ing4_Maltose
CHEBI_17306["CHEBI:17306"]
ing4_Maltose -.-> CHEBI_17306
ing5_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
CultureMech_002017 --> ing5_Na_acetate_x_3_H2O
CHEBI_32138["CHEBI:32138"]
ing5_Na_acetate_x_3_H2O -.-> CHEBI_32138
ing6__NH4_2_citrate["(NH4)2 citrate"]
CultureMech_002017 --> ing6__NH4_2_citrate
CHEBI_63076["CHEBI:63076"]
ing6__NH4_2_citrate -.-> CHEBI_63076
ing7_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_002017 --> ing7_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002017 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_002017 --> ing9_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing9_MnSO4_x_4_H2O -.-> CHEBI_86358
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002017 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Tween_80["Tween 80"]
CultureMech_002017 --> ing11_Tween_80
CHEBI_53426["CHEBI:53426"]
ing11_Tween_80 -.-> CHEBI_53426
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Meat extract | 3 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
4 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Maltose
C12H22O11 342.297 g/mol
|
4 G_PER_L |
CHEBI:17306
maltose
|
|
| 6 |
Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
|
5 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 7 |
(NH4)2 citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
|
| 8 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
2.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.05 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Preparation
- Adjust pH to 6.3 and fill up into screw-capped Wheaton tubes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.535324Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 859 |
| 2026-01-28T19:48:16.117201Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:09.440318Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002017 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.002459+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS MEDIUM IV" to "lactobacillus_medium_iv" for consistent matching |
| 2026-06-05T07:41:10.729189+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.473542+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |