halothiobacillus_medium

CultureMech:002023 bacterial DEFINED LIQUID
Original name: HALOTHIOBACILLUS MEDIUM

Overview

External ontology
mediadive.medium:864 (source link unavailable) — HALOTHIOBACILLUS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.5, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium864.pdf

Composition

flowchart LR
    CultureMech_002023["halothiobacillus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002023 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002023 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002023 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002023 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_002023 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Bromothymol_blue["Bromothymol blue"]
    CultureMech_002023 --> ing5_Bromothymol_blue
    CHEBI_86155["CHEBI:86155"]
    ing5_Bromothymol_blue -.-> CHEBI_86155
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002023 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002023 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_002023 --> ing8_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002023 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002023 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002023 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002023 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002023 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002023 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002023 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002023 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
28.8557 G_PER_L CHEBI:26710
sodium chloride
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.995025 G_PER_L CHEBI:62946
ammonium sulfate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.49254 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.41791 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
0.696517 G_PER_L CHEBI:32588
potassium chloride
6 Bromothymol blue
C27H28Br2O5S 624.391 g/mol
0.0039801 G_PER_L CHEBI:86155
bromothymol blue
7 K2HPO4
HO4P.2K 174.174 g/mol
0.497512 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
4.97512 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
5.2 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0.
  2. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.553949Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 864
2026-03-13T06:19:09.505745Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002023
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.074924+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOTHIOBACILLUS MEDIUM" to "halothiobacillus_medium" for consistent matching
2026-05-20T06:01:22.399802+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.5-7' -> {'min': 6.5, 'max': 7.0}
2026-06-05T07:41:08.266113+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.688249+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.