chloroflexus_aggregans_medium

Overview

External ontology

mediadive.medium:87a (source link unavailable) — CHLOROFLEXUS AGGREGANS MEDIUM

Applications

• Microbial cultivation

pH

8.2

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87a.pdf

Composition

flowchart LR
    CultureMech_002039["chloroflexus_aggregans_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002039 --> ing0_Yeast_extract
    ing1_Glycyl_glycine["Glycyl-glycine"]
    CultureMech_002039 --> ing1_Glycyl_glycine
    CHEBI_17201["CHEBI:17201"]
    ing1_Glycyl_glycine -.-> CHEBI_17201
    ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_002039 --> ing2_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002039 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KNO3["KNO3"]
    CultureMech_002039 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_NaNO3["NaNO3"]
    CultureMech_002039 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_NaCl["NaCl"]
    CultureMech_002039 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002039 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_002039 --> ing8_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing8_Fe_III__citrate -.-> CHEBI_144421
    ing9_Biotin["Biotin"]
    CultureMech_002039 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_002039 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002039 --> ing11_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing11_Calcium_pantothenate -.-> CHEBI_31345
    ing12_Na3_EDTA["Na3-EDTA"]
    CultureMech_002039 --> ing12_Na3_EDTA
    CHEBI_63125["CHEBI:63125"]
    ing12_Na3_EDTA -.-> CHEBI_63125
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002039 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002039 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_H3BO3["H3BO3"]
    CultureMech_002039 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002039 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002039 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002039 --> ing18_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002039 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002039 --> ing20_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing20_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (21)

Preparation

1. Adjust pH to 8.2.
2. Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.03% end concentration) to each bottle. This medium can be stored for several months. Incubate the culture at 50°C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366).
3. Adjust pH to 7.5. The solution is filter-sterilized.
4. The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.
5. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour.

Solutions

Target organisms

No target organisms are recorded.

Provenance