leptospirillum_hh_medium

CultureMech:002042 bacterial DEFINED LIQUID
Original name: LEPTOSPIRILLUM (HH) MEDIUM

Overview

External ontology
mediadive.medium:882 (source link unavailable) — LEPTOSPIRILLUM (HH) MEDIUM
Applications
  • Microbial cultivation
pH
2.3
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium882.pdf

Composition

flowchart LR
    CultureMech_002042["leptospirillum_hh_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002042 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002042 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002042 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002042 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002042 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_H2SO4["H2SO4"]
    CultureMech_002042 --> ing5_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing5_H2SO4 -.-> CHEBI_26836
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002042 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_ZnCl2["ZnCl2"]
    CultureMech_002042 --> ing7_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing7_ZnCl2 -.-> CHEBI_49976
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002042 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_H3BO3["H3BO3"]
    CultureMech_002042 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_Na2MoO4["Na2MoO4"]
    CultureMech_002042 --> ing10_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing10_Na2MoO4 -.-> CHEBI_75215
    ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002042 --> ing11_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing11_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.138947 G_PER_L CHEBI:62946
ammonium sulfate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0557895 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.0284211 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.154737 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
400 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 H2SO4
H2O4S 98.079 g/mol
50 G_PER_L CHEBI:26836
sulfuric acid
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.076 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 ZnCl2
2Cl.Zn 136.296 g/mol
0.068 G_PER_L CHEBI:49976
zinc dichloride
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.064 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid
11 Na2MoO4
MoO4.2Na 205.916 g/mol
0.01 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
12 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.067 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 2.3.
  2. Note: At the beginning of growth incubate statically without shaking.
  3. Adjust pH to 2.3 with 10 N H2SO4.
  4. The pH of this solution should be 1.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.611472Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 882
2026-03-13T06:19:09.718965Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002042
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.311000+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LEPTOSPIRILLUM (HH) MEDIUM" to "leptospirillum_hh_medium" for consistent matching
2026-06-05T07:41:11.106320+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.531033+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.