Overview
- External ontology
- mediadive.medium:885 (source link unavailable) — PHC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium885.pdf
Composition
flowchart LR
CultureMech_002045["phc_medium"]
ing0_Poly_hexamethylene_carbonate["Poly hexamethylene carbonate"]
CultureMech_002045 --> ing0_Poly_hexamethylene_carbonate
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002045 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_Yeast_extract["Yeast extract"]
CultureMech_002045 --> ing2_Yeast_extract
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002045 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_002045 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002045 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002045 --> ing6_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing7_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002045 --> ing7_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing7_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing8_MnSO4["MnSO4"]
CultureMech_002045 --> ing8_MnSO4
CHEBI_86360["CHEBI:86360"]
ing8_MnSO4 -.-> CHEBI_86360
ing9_Surfactant["Surfactant"]
CultureMech_002045 --> ing9_Surfactant
CHEBI_35195["CHEBI:35195"]
ing9_Surfactant -.-> CHEBI_35195
ing10_KH2PO4["KH2PO4"]
CultureMech_002045 --> ing10_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing10_KH2PO4 -.-> CHEBI_63036
ing11_K2HPO4["K2HPO4"]
CultureMech_002045 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Poly hexamethylene carbonate | 1 G_PER_L | ||
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 | Yeast extract | 0.1 G_PER_L | ||
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.01 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0005 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 8 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0005 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 9 |
MnSO4
Mn.O4S 151.001 g/mol
|
0.0005 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 10 | Surfactant | 0.06 G_PER_L |
CHEBI:35195
surfactant
|
|
| 11 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Preparation
- pH 7.0
- PHC: Emulsified, number-average molecular mass Mn=2000; Toagosei
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.618972Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 885 |
| 2026-03-13T06:19:09.742085Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002045 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.336348+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PHC MEDIUM" to "phc_medium" for consistent matching |
| 2026-06-05T07:41:41.732380+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.177234+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-20T19:03:01.472473+00:00 | apply_mim_groundings.py | Adopted MIM published grounding | Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308). |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |