aquaspirillum_medium

CultureMech:002048 bacterial COMPLEX SOLID_AGAR
Original name: AQUASPIRILLUM MEDIUM

Overview

External ontology
mediadive.medium:888 (source link unavailable) — AQUASPIRILLUM MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium888.pdf

Composition

flowchart LR
    CultureMech_002048["aquaspirillum_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002048 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002048 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_002048 --> ing2_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_002048 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_Sodium_succinate["Sodium succinate"]
    CultureMech_002048 --> ing4_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing4_Sodium_succinate -.-> CHEBI_63675
    ing5_Casamino_acids["Casamino acids"]
    CultureMech_002048 --> ing5_Casamino_acids
    ing6_Agar["Agar"]
    CultureMech_002048 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002048 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_EDTA["EDTA"]
    CultureMech_002048 --> ing8_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing8_EDTA -.-> CHEBI_4735
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002048 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002048 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002048 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002048 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002048 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002048 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002048 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002048 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Thiamine["Thiamine"]
    CultureMech_002048 --> ing17_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing17_Thiamine -.-> CHEBI_18385
    ing18_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002048 --> ing18_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing18_Calcium_pantothenate -.-> CHEBI_31345
    ing19_Biotin["Biotin"]
    CultureMech_002048 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_PABA["PABA"]
    CultureMech_002048 --> ing20_PABA
    CHEBI_30753["CHEBI:30753"]
    ing20_PABA -.-> CHEBI_30753
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002048 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Pyridoxine["Pyridoxine"]
    CultureMech_002048 --> ing22_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing22_Pyridoxine -.-> CHEBI_16709
    ing23_NaOH["NaOH"]
    CultureMech_002048 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Folic_acid["Folic acid"]
    CultureMech_002048 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Riboflavin["Riboflavin"]
    CultureMech_002048 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_B12["B12"]
    CultureMech_002048 --> ing26_B12

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.03 G_PER_L CHEBI:91243
calcium chloride hexahydrate
4 Na2HPO4
HO4P.2Na 141.958 g/mol
0.01 G_PER_L CHEBI:34683
disodium hydrogenphosphate
5 Sodium succinate
C4H4O4.2Na 162.052 g/mol
1 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
6 Casamino acids 1.5 G_PER_L
7 Agar 0.5 G_PER_L CHEBI:2509
agar
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.1 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Thiamine
C12H17N4OS 265.362 g/mol
0.05 G_PER_L CHEBI:18385
thiamine(1+)
19 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
21 PABA
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
23 Pyridoxine
C8H11NO3 169.18 g/mol
0.1 G_PER_L CHEBI:16709
pyridoxine
24 NaOH
HO.Na 39.997 g/mol
100 G_PER_L CHEBI:32145
sodium hydroxide
25 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
27 B12 0.005 G_PER_L

Preparation

  1. pH 7.5
  2. Add after autoclaving sterile solutions of:
  3. pH 3.0-4.0
  4. Sterilize by filtration.
  5. Mix the four solutions in the ratio 1:1:1:1.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.627950Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 888
2026-01-28T19:48:09.573495Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:09.774653Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002048
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.372018+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AQUASPIRILLUM MEDIUM" to "aquaspirillum_medium" for consistent matching
2026-06-05T07:40:49.393501+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.754394+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:53.412291+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.