iss1_medium

CultureMech:002049 bacterial COMPLEX LIQUID
Original name: ISS1 MEDIUM

Overview

External ontology
mediadive.medium:889 (source link unavailable) — ISS1 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium889.pdf

Composition

flowchart LR
    CultureMech_002049["iss1_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002049 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002049 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002049 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_002049 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_002049 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002049 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002049 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002049 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002049 --> ing8_Yeast_extract
    ing9_Sulfur["Sulfur"]
    CultureMech_002049 --> ing9_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing9_Sulfur -.-> CHEBI_26833
    ing10_NaHCO3["NaHCO3"]
    CultureMech_002049 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002049 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002049 --> ing12_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing12_MnSO4_x_H2O -.-> CHEBI_86364
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002049 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002049 --> ing14_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002049 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002049 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002049 --> ing17_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing18_H3BO3["H3BO3"]
    CultureMech_002049 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002049 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002049 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002049 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
21.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 20.0, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 7.0, 3.0]
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.65 G_PER_L CHEBI:32588
potassium chloride
5 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
6 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
7 K2HPO4
HO4P.2K 174.174 g/mol
0.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.5, 0.1]
9 Yeast extract 0.2 G_PER_L
10 Sulfur
S 32.067 g/mol
5 G_PER_L CHEBI:26833
sulfur atom
11 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
16 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
17 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
18 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Adjust pH to 7.0 using H2SO4.
  2. Prepare the medium under H2 gas and fill 20 ml medium into 100 ml serum bottles and seal with a rubber stopper. Steam/heat the medium in an almost boiling waterbath for 3 hours on each of 3 successive days to sterilize.
  3. Add bicarbonate from a autoclaved and neutralized stock solution.
  4. Check pH and adjust accordingly.
  5. Give an atmosphere of H2/CO2/O2 (78/20/2) with an overpressure of two atmospheres.
  6. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.631694Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 889
2026-03-13T03:09:38.826182Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:09.787023Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002049
2026-03-17T06:30:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.385373+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISS1 MEDIUM" to "iss1_medium" for consistent matching
2026-06-05T07:41:09.723244+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.157241+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.