Overview
- External ontology
- mediadive.medium:892 (source link unavailable) — METHANOCALCULUS PUMILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.3, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium892.pdf
Composition
flowchart LR
CultureMech_002055["methanocalculus_pumilus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002055 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_002055 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002055 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_NaCl["NaCl"]
CultureMech_002055 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na_acetate["Na-acetate"]
CultureMech_002055 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Yeast_extract["Yeast extract"]
CultureMech_002055 --> ing5_Yeast_extract
ing6_Trypticase_peptone["Trypticase peptone"]
CultureMech_002055 --> ing6_Trypticase_peptone
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_002055 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_002055 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002055 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002055 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002055 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002055 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002055 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002055 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002055 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_ZnCl2["ZnCl2"]
CultureMech_002055 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_CuCl2["CuCl2"]
CultureMech_002055 --> ing17_CuCl2
CHEBI_49553["CHEBI:49553"]
ing17_CuCl2 -.-> CHEBI_49553
ing18_H3BO3["H3BO3"]
CultureMech_002055 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002055 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002055 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002055 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002055 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing23_Biotin["Biotin"]
CultureMech_002055 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_002055 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002055 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_002055 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_002055 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_002055 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002055 --> ing29_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_D_____pantothenate -.-> CHEBI_31345
CultureMech_002055 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.395648 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.890208 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.356083 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
10.8912 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 9.8912, 1.0] |
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.49456 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Yeast extract | 1.97824 G_PER_L | ||
| 7 | Trypticase peptone | 1.97824 G_PER_L | ||
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
4.9456 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49456 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.49456 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.03 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.04 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients except carbonate, vitamins, cysteine and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve carbonate, adjust pH to 7.2 then dispense medium under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of their volume and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 7.3 - 7.5, if necessary.
- After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1.5 - 2 bar overpressure.
- First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.643014Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 892 |
| 2026-02-06T05:10:39.248115Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:20.203921Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:09.848294Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002055 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.452185+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOCALCULUS PUMILUS MEDIUM" to "methanocalculus_pumilus_medium" for consistent matching |
| 2026-05-20T06:01:55.286192+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5} |
| 2026-06-05T07:41:31.008419+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.775850+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |