Overview
- External ontology
- mediadive.medium:901 (source link unavailable) — ACIDOLOBUS ACETICUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 3.5, 'max': 3.8}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium901.pdf
Composition
flowchart LR
CultureMech_002067["acidolobus_aceticus_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002067 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KCl["KCl"]
CultureMech_002067 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_KH2PO4["KH2PO4"]
CultureMech_002067 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002067 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002067 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_002067 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_Sulfur["Sulfur"]
CultureMech_002067 --> ing6_Sulfur
CHEBI_26833["CHEBI:26833"]
ing6_Sulfur -.-> CHEBI_26833
ing7_Yeast_extract["Yeast extract"]
CultureMech_002067 --> ing7_Yeast_extract
ing8_HCl["HCl"]
CultureMech_002067 --> ing8_HCl
CHEBI_17883["CHEBI:17883"]
ing8_HCl -.-> CHEBI_17883
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002067 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_ZnCl2["ZnCl2"]
CultureMech_002067 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002067 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_H3BO3["H3BO3"]
CultureMech_002067 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002067 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002067 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002067 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002067 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_Biotin["Biotin"]
CultureMech_002067 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_002067 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002067 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_002067 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_Riboflavin["Riboflavin"]
CultureMech_002067 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_002067 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002067 --> ing23_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_002067 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002067 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_002067 --> ing26__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing27_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002067 --> ing27_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing27_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.324484 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.324484 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.324484 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.324484 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.324484 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000491642 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
Sulfur
S 32.067 g/mol
|
9.83284 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 8 | Yeast extract | 2.94985 G_PER_L | ||
| 9 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 28 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients except sulfur, yeast extract, vitamins and sulfide. Adjust pH to 3.5 with H2SO4 and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of sulfur. Heat vessels containing medium to 90°C for 1 hour on each of 3 succesive days. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The sulfide stock solution (3% w/v) should be neutralized before use (see medium 29). The pH of the complete medium should be at 3.5 - 3.8.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.684088Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 901 |
| 2026-02-06T05:10:32.618396Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:09.981428Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002067 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.595374+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDOLOBUS ACETICUS MEDIUM" to "acidolobus_aceticus_medium" for consistent matching |
| 2026-05-20T06:01:07.536603+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '3.5-3.8' -> {'min': 3.5, 'max': 3.8} |
| 2026-06-05T07:40:47.410865+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.637066+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_901_acidolobus_score10.py | RESOLVED_KOMODO_901_ACIDOLOBUS_TOPOLOGY | Pointed the KOMODO Medium 901 child link at the archaeal file. |