methanocalculus_halotolerans_medium

CultureMech:002073 archaea COMPLEX LIQUID
Original name: METHANOCALCULUS HALOTOLERANS MEDIUM

Overview

External ontology
mediadive.medium:905 (source link unavailable) — METHANOCALCULUS HALOTOLERANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.6}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium905.pdf

Composition

flowchart LR
    CultureMech_002073["methanocalculus_halotolerans_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_002073 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002073 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_002073 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_002073 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaCl["NaCl"]
    CultureMech_002073 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Na_acetate["Na-acetate"]
    CultureMech_002073 --> ing5_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Na_acetate -.-> CHEBI_32954
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002073 --> ing6_Yeast_extract
    ing7_Trypticase["Trypticase"]
    CultureMech_002073 --> ing7_Trypticase
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_002073 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002073 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002073 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002073 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002073 --> ing12_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002073 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002073 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002073 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002073 --> ing16_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_x_H2O -.-> CHEBI_86364
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002073 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002073 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002073 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002073 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_002073 --> ing21_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_H3BO3["H3BO3"]
    CultureMech_002073 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002073 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002073 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002073 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002073 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.990099 G_PER_L CHEBI:31206
ammonium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.29703 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.29703 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 KCl
Cl.K 74.551 g/mol
0.168317 G_PER_L CHEBI:32588
potassium chloride
5 NaCl
Cl.Na 58.443 g/mol
50.505 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 49.505, 1.0]
6 Na-acetate
C2H3O2.Na 82.034 g/mol
0.49505 G_PER_L CHEBI:32954
sodium acetate
7 Yeast extract 0.49505 G_PER_L
8 Trypticase 0.49505 G_PER_L MICRO:0000175 (source link unavailable)
Trypticase peptone
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049505 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
1.9802 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49505 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.694059 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.594059, 0.1]
13 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3.16832 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.29703 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Dissolve ingredients (except bicarbonate, cysteine, calcium chloride, magnesium chloride and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve solid bicarbonate and cysteine, then adjust pH to 7.5 with 10 N KOH. Dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add calcium chloride, magnesium chloride and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be between 7.2 and 7.6.
  2. After inoculation pressurize vessels with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.705039Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 905
2026-01-28T19:48:27.185208Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:15.428711Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:10.043780Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002073
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.664946+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOCALCULUS HALOTOLERANS MEDIUM" to "methanocalculus_halotolerans_medium" for consistent matching
2026-05-20T06:01:55.233721+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.6' -> {'min': 7.2, 'max': 7.6}
2026-06-05T07:41:30.943870+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.751259+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-22T00:03:26.686152+00:00 repair_rejected_peptone_groundings.py CORRECTED_REJECTED_PEPTONE_GROUNDING CultureMech #484. /ingredients/7/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.