Overview
- External ontology
- mediadive.medium:908 (source link unavailable) — MEDIUM FOR CHLORATE RESPIRERS
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium908.pdf
Composition
flowchart LR
CultureMech_002076["medium_for_chlorate_respirers"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002076 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_NaH2PO4["NaH2PO4"]
CultureMech_002076 --> ing1_NaH2PO4
CHEBI_37585["CHEBI:37585"]
ing1_NaH2PO4 -.-> CHEBI_37585
ing2_KCl["KCl"]
CultureMech_002076 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NaHCO3["NaHCO3"]
CultureMech_002076 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_NaClO3["NaClO3"]
CultureMech_002076 --> ing4_NaClO3
CHEBI_65242["CHEBI:65242"]
ing4_NaClO3 -.-> CHEBI_65242
ing5_Na_acetate["Na-acetate"]
CultureMech_002076 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_MgSO4["MgSO4"]
CultureMech_002076 --> ing6_MgSO4
CHEBI_32599["CHEBI:32599"]
ing6_MgSO4 -.-> CHEBI_32599
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002076 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Na2MoO4["Na2MoO4"]
CultureMech_002076 --> ing8_Na2MoO4
CHEBI_75215["CHEBI:75215"]
ing8_Na2MoO4 -.-> CHEBI_75215
ing9_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002076 --> ing9_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing9_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing10_HCl["HCl"]
CultureMech_002076 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002076 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_002076 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002076 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_002076 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002076 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002076 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002076 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002076 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_002076 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_Pantothenic_acid["Pantothenic acid"]
CultureMech_002076 --> ing20_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing20_Pantothenic_acid -.-> CHEBI_7916
ing21_Riboflavin["Riboflavin"]
CultureMech_002076 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_002076 --> ing22_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing22_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing23_Biotin["Biotin"]
CultureMech_002076 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_002076 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002076 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Nicotine_amide["Nicotine amide"]
CultureMech_002076 --> ing26_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing26_Nicotine_amide -.-> CHEBI_17154
ing27_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_002076 --> ing27_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_alpha_lipoic_acid -.-> CHEBI_16494
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002076 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_002076 --> ing29_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing29_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
NaH2PO4
H2O4P.Na 119.976 g/mol
|
0.6 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
NaClO3
ClO3.Na 106.44 g/mol
|
1 G_PER_L |
CHEBI:65242
sodium chlorate
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1.36 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 |
MgSO4
Mg.O4S 120.368 g/mol
|
3 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Na2MoO4
MoO4.2Na 205.916 g/mol
|
0.025 G_PER_L |
CHEBI:75215
sodium molybdate (anhydrous)
|
|
| 10 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.025 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 28 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- Autoclave solutions A, B and C separately and anaerobically under 80% N2 + 20% CO2 atmosphere. Combine 1000 ml solution A, 10 ml solution B and 10 ml solution C. Add 5 ml vitamin solution E and 1 ml trace element solution D. The pH should be 7.2 without further adjustment.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
- Stir for some hours, filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.713702Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 908 |
| 2026-01-28T19:48:16.496513Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:10.069063Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002076 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.694545+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM FOR CHLORATE RESPIRERS" to "medium_for_chlorate_respirers" for consistent matching |
| 2026-06-05T07:41:28.952493+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.013386+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |