ams_medium

CultureMech:002091 bacterial COMPLEX SOLID_AGAR
Original name: AMS medium

Overview

External ontology
mediadive.medium:921a (source link unavailable) — AMS medium
Applications
  • Microbial cultivation
Notes
Source: DSMZ

Composition

flowchart LR
    CultureMech_002091["ams_medium"]
    ing0_Agar["Agar"]
    CultureMech_002091 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Na2_EDTA["Na2-EDTA"]
    CultureMech_002091 --> ing1_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing1_Na2_EDTA -.-> CHEBI_64734
    ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002091 --> ing2_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002091 --> ing3_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing4_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002091 --> ing4_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing4_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing5_H3BO3["H3BO3"]
    CultureMech_002091 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002091 --> ing6_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002091 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002091 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_002091 --> ing9_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing9_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing10_KH2PO4["KH2PO4"]
    CultureMech_002091 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_002091 --> ing11_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing11_Fe_III__EDTA -.-> CHEBI_30729
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002091 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_NH4Cl["NH4Cl"]
    CultureMech_002091 --> ing13_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing13_NH4Cl -.-> CHEBI_31206
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002091 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002091 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
2 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
3 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
4 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
5 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
6 H3BO3
H3BO3 61.833 g/mol
0.015 G_PER_L CHEBI:33118
boric acid
7 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.05 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.02 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
10 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
71.6 G_PER_L CHEBI:34683
disodium hydrogenphosphate
11 KH2PO4
H2O4P.K 136.084 g/mol
26 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
3.8 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.26 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 NH4Cl
Cl.H4N 53.492 g/mol
0.53 G_PER_L CHEBI:31206
ammonium chloride
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate

Preparation

  1. Combine Solutions 1-3 with Trace Elements and distilled water. Then dispense into growth vessels and autoclave under 20% methane in air (or strain specific other ratio) atmosphere (if not using agar).
  2. Add separately autoclaved phosphate buffer (10 ml/L) to autoclaved and cooled medium. If phosphate buffer is added to warm medium, it will precipitate.
  3. Liquid cultures should be grown with shaking.
  4. May be stored at 4°C in the dark
  5. Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.757002Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 921a
2026-03-13T06:19:10.201364Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002091
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.847560+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AMS medium" to "ams_medium" for consistent matching
2026-06-05T07:40:48.665733+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.085493+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.