methylocapsa_acidophila_medium

CultureMech:002092 bacterial DEFINED LIQUID
Original name: METHYLOCAPSA ACIDOPHILA MEDIUM

Overview

External ontology
mediadive.medium:922 (source link unavailable) — METHYLOCAPSA ACIDOPHILA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 4.0, 'max': 4.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium922.pdf

Composition

flowchart LR
    CultureMech_002092["methylocapsa_acidophila_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002092 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002092 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002092 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KNO3["KNO3"]
    CultureMech_002092 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_EDTA["EDTA"]
    CultureMech_002092 --> ing4_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing4_EDTA -.-> CHEBI_4735
    ing5_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_002092 --> ing5_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing5_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002092 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002092 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002092 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002092 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_Na2MoO4["Na2MoO4"]
    CultureMech_002092 --> ing10_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing10_Na2MoO4 -.-> CHEBI_75215

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
 (enhances growth)
5 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
6 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.1 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 Na2MoO4
MoO4.2Na 205.916 g/mol
0.03 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)

Preparation

  1. Final pH 4.0-4.5.
  2. The medium is fairly weakly buffered so the pH should be checked before and after autoclaving. In the early stage of growth the strain may form aggregates which break as the culture ages. 10-30% methane is added to the gas phase. Adding 100 mg/litre KNO3 enhances growth.
  3. The strain should be grown with shaking.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.759831Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 922
2026-03-13T06:19:10.208303Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002092
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.856171+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOCAPSA ACIDOPHILA MEDIUM" to "methylocapsa_acidophila_medium" for consistent matching
2026-05-20T06:01:56.725734+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '4-4.5' -> {'min': 4.0, 'max': 4.5}
2026-06-05T07:41:33.047754+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.511823+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.527779+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.