alkalibacterium_olivapovliticus

CultureMech:002093 bacterial COMPLEX SOLID_AGAR
Original name: ALKALIBACTERIUM OLIVAPOVLITICUS

Overview

External ontology
mediadive.medium:923 (source link unavailable) — ALKALIBACTERIUM OLIVAPOVLITICUS
Applications
  • Microbial cultivation
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium923.pdf

Composition

flowchart LR
    CultureMech_002093["alkalibacterium_olivapovliticus"]
    ing0_Na_glutamate["Na glutamate"]
    CultureMech_002093 --> ing0_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing0_Na_glutamate -.-> CHEBI_64220
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002093 --> ing1_Yeast_extract
    ing2_Na2CO3["Na2CO3"]
    CultureMech_002093 --> ing2_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing2_Na2CO3 -.-> CHEBI_29377
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002093 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002093 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002093 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Agar["Agar"]
    CultureMech_002093 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Na glutamate
C5H8NO4.Na 169.112 g/mol
10 G_PER_L CHEBI:64220
monosodium glutamate
2 Yeast extract 5 G_PER_L
3 Na2CO3
CO3.2Na 105.988 g/mol
10 G_PER_L CHEBI:29377
sodium carbonate
4 K2HPO4
HO4P.2K 174.174 g/mol
0.15 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.025 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1 G_PER_L CHEBI:62946
ammonium sulfate
7 Agar 20 G_PER_L CHEBI:2509
agar
(for solid medium)

Preparation

  1. We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.762073Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 923
2026-03-13T06:19:10.213476Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002093
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.862120+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALIBACTERIUM OLIVAPOVLITICUS" to "alkalibacterium_olivapovliticus" for consistent matching
2026-06-05T07:40:47.810266+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.770930+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T12:21:30.028294+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.