Overview
- External ontology
- mediadive.medium:923 (source link unavailable) — ALKALIBACTERIUM OLIVAPOVLITICUS
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium923.pdf
Composition
flowchart LR
CultureMech_002093["alkalibacterium_olivapovliticus"]
ing0_Na_glutamate["Na glutamate"]
CultureMech_002093 --> ing0_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing0_Na_glutamate -.-> CHEBI_64220
ing1_Yeast_extract["Yeast extract"]
CultureMech_002093 --> ing1_Yeast_extract
ing2_Na2CO3["Na2CO3"]
CultureMech_002093 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_K2HPO4["K2HPO4"]
CultureMech_002093 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002093 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_002093 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Agar["Agar"]
CultureMech_002093 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
10 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.15 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.025 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.762073Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 923 |
| 2026-03-13T06:19:10.213476Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002093 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.862120+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIBACTERIUM OLIVAPOVLITICUS" to "alkalibacterium_olivapovliticus" for consistent matching |
| 2026-06-05T07:40:47.810266+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.770930+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T12:21:30.028294+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |