halorhabdus_utahensis_medium

CultureMech:002099 archaea COMPLEX LIQUID
Original name: HALORHABDUS UTAHENSIS MEDIUM

Overview

External ontology
mediadive.medium:927 (source link unavailable) — HALORHABDUS UTAHENSIS MEDIUM
Applications
  • Microbial cultivation
pH
7.6
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium927.pdf

Composition

flowchart LR
    CultureMech_002099["halorhabdus_utahensis_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002099 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NaBr["NaBr"]
    CultureMech_002099 --> ing1_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing1_NaBr -.-> CHEBI_63004
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002099 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002099 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002099 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Tris_HCl["Tris-HCl"]
    CultureMech_002099 --> ing5_Tris_HCl
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002099 --> ing6_Yeast_extract
    ing7_Glucose["Glucose"]
    CultureMech_002099 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_HCl["HCl"]
    CultureMech_002099 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_7_H2O["FeCl2 x 7 H2O"]
    CultureMech_002099 --> ing9_FeCl2_x_7_H2O
    CHEBI_30812["CHEBI:30812"]
    ing9_FeCl2_x_7_H2O -.-> CHEBI_30812
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002099 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002099 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002099 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002099 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002099 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002099 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002099 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_KH2PO4["KH2PO4"]
    CultureMech_002099 --> ing17_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing17_KH2PO4 -.-> CHEBI_63036
    ing18_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_002099 --> ing18_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing18_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing19_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002099 --> ing19_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing19_FeCl2_x_4_H2O -.-> CHEBI_86249

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
270 G_PER_L CHEBI:26710
sodium chloride
2 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
6 Tris-HCl 12 G_PER_L
7 Yeast extract 1 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
9 HCl
HCl 36.461 g/mol
6.5 G_PER_L CHEBI:17883
hydrogen chloride
10 FeCl2 x 7 H2O
Cl2Fe 126.751 g/mol
1.5 G_PER_L CHEBI:30812
iron dichloride
11 ZnCl2
2Cl.Zn 136.296 g/mol
70 MG_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
100 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Unit conflict resolved: assumed G_PER_L was data entry error, kept MG_PER_L]
13 H3BO3
H3BO3 61.833 g/mol
62 MG_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
190 MG_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
17 MG_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
24 MG_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
36 MG_PER_L CHEBI:75213
sodium molybdate dihydrate
18 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
19 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
100 G_PER_L CHEBI:91243
calcium chloride hexahydrate
20 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
20 G_PER_L CHEBI:86249
iron dichloride tetrahydrate

Preparation

  1. Prepare the medium, and adjust the pH to 7.6 with 5M NaOH.
  2. Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372!
  3. Prepare and sterilise separately
  4. Prepare and sterilise separately
  5. Prepare and sterilise separately

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.785372Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 927
2026-02-06T05:10:22.239313Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:53.707591Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s); WARNING: Duplicate 'MnCl2 x 4 H2O' with different units: {'MG_PER_L', 'G_PER_L'}
2026-03-13T04:23:43.705438Z unit-conflict-resolver-v1.0 Resolved unit conflicts Resolved trace metal unit conflict for MnCl2 x 4 H2O
2026-03-13T06:19:10.293979Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002099
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.948837+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALORHABDUS UTAHENSIS MEDIUM" to "halorhabdus_utahensis_medium" for consistent matching
2026-06-05T07:41:08.125058+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.633964+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.