rhodovulum_iodosum_and_r_robiginosum_medium

CultureMech:002101 bacterial DEFINED LIQUID
Original name: RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM

Overview

External ontology
mediadive.medium:929 (source link unavailable) — RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium929.pdf

Composition

flowchart LR
    CultureMech_002101["rhodovulum_iodosum_and_r_robiginosum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002101 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002101 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002101 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002101 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_002101 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KBr["KBr"]
    CultureMech_002101 --> ing5_KBr
    CHEBI_32030["CHEBI:32030"]
    ing5_KBr -.-> CHEBI_32030
    ing6_KH2PO4["KH2PO4"]
    CultureMech_002101 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NH4Cl["NH4Cl"]
    CultureMech_002101 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002101 --> ing8_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing9_NaHCO3["NaHCO3"]
    CultureMech_002101 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Sodium_acetate["Sodium acetate"]
    CultureMech_002101 --> ing10_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing10_Sodium_acetate -.-> CHEBI_32954
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002101 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_Na2_EDTA["Na2-EDTA"]
    CultureMech_002101 --> ing12_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing12_Na2_EDTA -.-> CHEBI_64734
    ing13_H3BO3["H3BO3"]
    CultureMech_002101 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002101 --> ing14_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002101 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002101 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002101 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002101 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002101 --> ing19_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002101 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002101 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_NaOH["NaOH"]
    CultureMech_002101 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Para_aminobenzoic_acid["Para-aminobenzoic acid"]
    CultureMech_002101 --> ing23_Para_aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing23_Para_aminobenzoic_acid -.-> CHEBI_30753
    ing24_Biotin["Biotin"]
    CultureMech_002101 --> ing24_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing24_Biotin -.-> CHEBI_15956
    ing25_Nicotinate["Nicotinate"]
    CultureMech_002101 --> ing25_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinate -.-> CHEBI_15940
    ing26_Pantothenate["Pantothenate"]
    CultureMech_002101 --> ing26_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing26_Pantothenate -.-> CHEBI_16454
    ing27_Pyridoxin["Pyridoxin"]
    CultureMech_002101 --> ing27_Pyridoxin
    ing28_Thiamin["Thiamin"]
    CultureMech_002101 --> ing28_Thiamin
    CHEBI_18385["CHEBI:18385"]
    ing28_Thiamin -.-> CHEBI_18385
    ing29_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_002101 --> ing29_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing29_Cyanocobalamine -.-> CHEBI_17439

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
26.4 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.7 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
0.66 G_PER_L CHEBI:32588
potassium chloride
6 KBr
Br.K 119.002 g/mol
0.09 G_PER_L CHEBI:32030
potassium bromide
7 KH2PO4
H2O4P.K 136.084 g/mol
0.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 NH4Cl
Cl.H4N 53.492 g/mol
0.25 G_PER_L CHEBI:31206
ammonium chloride
9 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.124 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
10 NaHCO3
CHO3.Na 84.006 g/mol
2.52 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.41015 G_PER_L CHEBI:32954
sodium acetate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
4.88015 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 2.78015, 2.1]
13 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
5.2 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
14 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
15 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 NaOH
HO.Na 39.997 g/mol
0.2 G_PER_L CHEBI:32145
sodium hydroxide
24 Para-aminobenzoic acid
C7H7NO2 137.138 g/mol
0.04 G_PER_L CHEBI:30753
4-aminobenzoic acid
25 Biotin
C10H16N2O3S 244.316 g/mol
0.01 G_PER_L CHEBI:15956
biotin
26 Nicotinate
C6H5NO2 123.111 g/mol
0.1 G_PER_L CHEBI:15940
nicotinic acid
27 Pantothenate
C9H16NO5 218.229 g/mol
0.05 G_PER_L CHEBI:16454
pantothenate
28 Pyridoxin 0.15 G_PER_L
29 Thiamin
C12H17N4OS 265.362 g/mol
0.1 G_PER_L CHEBI:18385
thiamine(1+)
30 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
0.05 G_PER_L CHEBI:17439
cyanocob(III)alamin

Preparation

  1. Sparge medium with 80% N2 and 20% CO2 gas mixture to make anoxic.
  2. Bring to the boil.
  3. Cool down under N2 atmosphere.
  4. Add bicarbonate.
  5. Adjust pH to 6.8.
  6. Dispense into appropriate vessels, e.g. Hungate tubes, under N2 atmosphere
  7. Autoclave at 121 °C for 15 min.
  8. After autoclaving or before use add the following filter-sterilzed solutions (to the given final concentrations).
  9. Note: When the iron is added to the medium a white floculant precipitate may appear. In the initial stages of growth the precipitate turns orange/brown as the ferrous ions are oxidised to ferrihydrite.
  10. Adjust the pH to 6.0

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.791827Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 929
2026-02-06T05:10:24.551260Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:08:57.204554Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:10.316729Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002101
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:22.974100+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM" to "rhodovulum_iodosum_and_r_robiginosum_medium" for consistent matching
2026-06-05T07:41:44.473761+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.011788+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.