Overview
- External ontology
- mediadive.medium:930 (source link unavailable) — NATRONINCOLA HISTIDINOVORANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.9
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium930.pdf
Composition
flowchart LR
CultureMech_002103["natronincola_histidinovorans_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002103 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002103 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_NH4Cl["NH4Cl"]
CultureMech_002103 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_002103 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_002103 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2CO3["Na2CO3"]
CultureMech_002103 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_NaHCO3["NaHCO3"]
CultureMech_002103 --> ing6_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing6_NaHCO3 -.-> CHEBI_32139
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002103 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
ing8_Yeast_extract["Yeast extract"]
CultureMech_002103 --> ing8_Yeast_extract
ing9_Histidine["Histidine"]
CultureMech_002103 --> ing9_Histidine
CHEBI_27570["CHEBI:27570"]
ing9_Histidine -.-> CHEBI_27570
ing10_Resazurin["Resazurin"]
CultureMech_002103 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Biotin["Biotin"]
CultureMech_002103 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002103 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002103 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002103 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002103 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002103 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002103 --> ing17_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_D_____pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002103 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002103 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_002103 --> ing20__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002103 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002103 --> ing22_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing22_MgSO4_x_7_H2O -.-> CHEBI_31795
ing23_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_002103 --> ing23_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing23_MnSO4_x_H2O -.-> CHEBI_86364
ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002103 --> ing24_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002103 --> ing25_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
ing26_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002103 --> ing26_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing26_CaCl2_x_2_H2O -.-> CHEBI_86158
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002103 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002103 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002103 --> ing29_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing29_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
CultureMech_002103 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
81.0 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 80.0, 1.0] |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6.83 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 7 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3.83 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.2 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 9 | Yeast extract | 0.2 G_PER_L | ||
| 10 |
Histidine
C6H9N3O2 155.157 g/mol
|
5 G_PER_L |
CHEBI:27570
histidine
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 23 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 24 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 25 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 26 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 27 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 31 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 32 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 33 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 34 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Final pH 8.9
- Prepare the medium without adding the vitamins, histidine, yeast extract, NH4Cl, Na2CO3, and NaHCO3 using anaerobic conditions, under nitrogen. If the medium has been boiled to remove oxygen add the NH4Cl, Na2CO3, and NaHCO3 after the medium had cooled. Dispense into tubes stopper with rubber stoppers (serum tubes or bottles, or screw capped tubes). Autoclave, and to the cooled medium add the vitamins (filter sterilised under N2), histidine, yeast extract, and Na2S x 9 H2O from anaerobic, sterile stock solutions.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.796394Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 930 |
| 2026-02-06T05:10:18.803613Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:48.474855Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:10.338003Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002103 |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:22.997935+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRONINCOLA HISTIDINOVORANS MEDIUM" to "natronincola_histidinovorans_medium" for consistent matching |
| 2026-06-05T07:41:39.318121+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.422822+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |