thermococcus_waiotapuense_medium

CultureMech:002107 archaea COMPLEX LIQUID
Original name: THERMOCOCCUS WAIOTAPUENSE MEDIUM

Overview

External ontology
mediadive.medium:934 (source link unavailable) — THERMOCOCCUS WAIOTAPUENSE MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium934.pdf

Composition

flowchart LR
    CultureMech_002107["thermococcus_waiotapuense_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002107 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Na2SO4["Na2SO4"]
    CultureMech_002107 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_KCl["KCl"]
    CultureMech_002107 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NaHCO3["NaHCO3"]
    CultureMech_002107 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_KBr["KBr"]
    CultureMech_002107 --> ing4_KBr
    CHEBI_32030["CHEBI:32030"]
    ing4_KBr -.-> CHEBI_32030
    ing5_H3BO3["H3BO3"]
    CultureMech_002107 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_MgCl2["MgCl2"]
    CultureMech_002107 --> ing6_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing6_MgCl2 -.-> CHEBI_6636
    ing7_CaCl2["CaCl2"]
    CultureMech_002107 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_SrCl2["SrCl2"]
    CultureMech_002107 --> ing8_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing8_SrCl2 -.-> CHEBI_36383
    ing9_Tryptone["Tryptone"]
    CultureMech_002107 --> ing9_Tryptone
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_002107 --> ing10_Yeast_extract
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002107 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Resazurin["Resazurin"]
    CultureMech_002107 --> ing12_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing12_Resazurin -.-> CHEBI_8806
    ing13_Sulphur["Sulphur"]
    CultureMech_002107 --> ing13_Sulphur
    CHEBI_17909["CHEBI:17909"]
    ing13_Sulphur -.-> CHEBI_17909

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1.5 G_PER_L CHEBI:26710
sodium chloride
2 Na2SO4
2Na.O4S 142.043 g/mol
4 G_PER_L CHEBI:32149
sodium sulfate
3 KCl
Cl.K 74.551 g/mol
0.7 G_PER_L CHEBI:32588
potassium chloride
4 NaHCO3
CHO3.Na 84.006 g/mol
0.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 KBr
Br.K 119.002 g/mol
0.1 G_PER_L CHEBI:32030
potassium bromide
6 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
7 MgCl2
2Cl.Mg 95.211 g/mol
5.8 G_PER_L CHEBI:6636
magnesium dichloride
8 CaCl2
Ca.2Cl 110.984 g/mol
1.5 G_PER_L CHEBI:3312
calcium dichloride
9 SrCl2
2Cl.Sr 158.526 g/mol
0.025 G_PER_L CHEBI:36383
strontium dichloride
10 Tryptone 5 G_PER_L
11 Yeast extract 1 G_PER_L
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.75 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Resazurin
C12H7NO4 229.191 g/mol
0.002 G_PER_L CHEBI:8806
Resazurin
14 Sulphur
(S)n.S2 96.201 g/mol
10 G_PER_L CHEBI:17909
polysulfur

Preparation

  1. pH 7.0
  2. Prepare the medium anaerobically under N2. Steam the medium for 3 hours (in a boiling water bath) on three successive days. Alternatively, sterilize in an autoclave at 110°C for 20 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.813704Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 934
2026-03-13T06:19:10.392993Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002107
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.054411+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOCOCCUS WAIOTAPUENSE MEDIUM" to "thermococcus_waiotapuense_medium" for consistent matching
2026-06-05T07:41:49.971328+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:12.938538+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.