Overview
- External ontology
- mediadive.medium:93 (source link unavailable) — LACTOBACILLUS MEDIUM II
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium93.pdf
Composition
flowchart LR
CultureMech_002115["lactobacillus_medium_ii"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_002115 --> ing0_Casein_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002115 --> ing1_Yeast_extract
ing2_Meat_extract["Meat extract"]
CultureMech_002115 --> ing2_Meat_extract
ing3_Glucose["Glucose"]
CultureMech_002115 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_KH2PO4["KH2PO4"]
CultureMech_002115 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_002115 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002115 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002115 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_002115 --> ing8_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing8_MnSO4_x_H2O -.-> CHEBI_86364
ing9_Tween_80["Tween 80"]
CultureMech_002115 --> ing9_Tween_80
CHEBI_53426["CHEBI:53426"]
ing9_Tween_80 -.-> CHEBI_53426
ing10_Ethanol["Ethanol"]
CultureMech_002115 --> ing10_Ethanol
CHEBI_16236["CHEBI:16236"]
ing10_Ethanol -.-> CHEBI_16236
ing11_Na_acetate["Na-acetate"]
CultureMech_002115 --> ing11_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Na_acetate -.-> CHEBI_32954
ing12_DL_mevalonic_acid["DL-mevalonic acid"]
CultureMech_002115 --> ing12_DL_mevalonic_acid
CHEBI_25351["CHEBI:25351"]
ing12_DL_mevalonic_acid -.-> CHEBI_25351
ing13_Agar["Agar"]
CultureMech_002115 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 10 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 | Meat extract | 2 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.0075 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 10 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 11 |
Ethanol
C2H6O 46.069 g/mol
|
40 G_PER_L |
CHEBI:16236
ethanol
|
|
| 12 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
20 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 13 |
DL-mevalonic acid
C6H12O4 148.158 g/mol
|
0.03 G_PER_L |
CHEBI:25351
mevalonic acid acid
|
|
| 14 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 5.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.159153Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 93 |
| 2026-03-13T06:19:10.452727Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002115 |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.120609+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS MEDIUM II" to "lactobacillus_medium_ii" for consistent matching |
| 2026-06-05T07:41:10.712740+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.466810+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |