Overview
- External ontology
- mediadive.medium:944 (source link unavailable) — PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium944.pdf
Composition
flowchart LR
CultureMech_002120["pseudomonas_chlorotidismutans_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002120 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4["Na2HPO4"]
CultureMech_002120 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_NaClO3["NaClO3"]
CultureMech_002120 --> ing2_NaClO3
CHEBI_65242["CHEBI:65242"]
ing2_NaClO3 -.-> CHEBI_65242
ing3_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
CultureMech_002120 --> ing3_Na_acetate_x_3_H2O
CHEBI_32138["CHEBI:32138"]
ing3_Na_acetate_x_3_H2O -.-> CHEBI_32138
ing4_Resazurin["Resazurin"]
CultureMech_002120 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_CaCl2["CaCl2"]
CultureMech_002120 --> ing5_CaCl2
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2 -.-> CHEBI_3312
ing6_MgCl2["MgCl2"]
CultureMech_002120 --> ing6_MgCl2
CHEBI_6636["CHEBI:6636"]
ing6_MgCl2 -.-> CHEBI_6636
ing7_NH4HCO3["NH4HCO3"]
CultureMech_002120 --> ing7_NH4HCO3
CHEBI_184335["CHEBI:184335"]
ing7_NH4HCO3 -.-> CHEBI_184335
ing8_NaHCO3["NaHCO3"]
CultureMech_002120 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002120 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_HCl["HCl"]
CultureMech_002120 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002120 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_ZnCl2["ZnCl2"]
CultureMech_002120 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002120 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_002120 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002120 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002120 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002120 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002120 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NaOH["NaOH"]
CultureMech_002120 --> ing19_NaOH
CHEBI_32145["CHEBI:32145"]
ing19_NaOH -.-> CHEBI_32145
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002120 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002120 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_002120 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_Pantothenic_acid["Pantothenic acid"]
CultureMech_002120 --> ing23_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing23_Pantothenic_acid -.-> CHEBI_7916
ing24_Riboflavin["Riboflavin"]
CultureMech_002120 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_002120 --> ing25_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing25_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing26_Biotin["Biotin"]
CultureMech_002120 --> ing26_Biotin
CHEBI_15956["CHEBI:15956"]
ing26_Biotin -.-> CHEBI_15956
ing27_Folic_acid["Folic acid"]
CultureMech_002120 --> ing27_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing27_Folic_acid -.-> CHEBI_27470
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_002120 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Nicotine_amide["Nicotine amide"]
CultureMech_002120 --> ing29_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing29_Nicotine_amide -.-> CHEBI_17154
CultureMech_002120 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.455556 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.588889 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
NaClO3
ClO3.Na 106.44 g/mol
|
1.17778 G_PER_L |
CHEBI:65242
sodium chlorate
|
|
| 4 |
Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
|
3.02222 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000555556 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
CaCl2
Ca.2Cl 110.984 g/mol
|
2.2 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
MgCl2
2Cl.Mg 95.211 g/mol
|
2 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 8 |
NH4HCO3
CHO3.H4N 79.055 g/mol
|
8.8 G_PER_L |
CHEBI:184335
Ammonium bicarbonate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
74.6 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.039 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.036, 0.003] |
| 20 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.003 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 27 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 28 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 31 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
Preparation
- All solutions are prepared anaerobically under N2 and autoclaved. After cooling, the solutions are combined under N2 gas. Add 1 ml/l vitamin solution. pH should be 8.5 without further adjustment.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
- Stir for some hours, filter sterilize the solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.844499Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 944 |
| 2026-01-28T19:48:20.269327Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:03.053097Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:10.496770Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002120 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.169810+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PSEUDOMONAS CHLOROTIDISMUTANS MEDIUM" to "pseudomonas_chlorotidismutans_medium" for consistent matching |
| 2026-06-05T07:41:42.715896+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.465191+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |