Overview
- External ontology
- mediadive.medium:946 (source link unavailable) — NAUTILIA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium946.pdf
Composition
flowchart LR
CultureMech_002124["nautilia_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_002124 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_002124 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Sodium_resazurin["Sodium resazurin"]
CultureMech_002124 --> ing2_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Sodium_resazurin -.-> CHEBI_8806
ing3_Sulfur["Sulfur"]
CultureMech_002124 --> ing3_Sulfur
CHEBI_26833["CHEBI:26833"]
ing3_Sulfur -.-> CHEBI_26833
ing4_Na2CO3["Na2CO3"]
CultureMech_002124 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Na_formate["Na-formate"]
CultureMech_002124 --> ing5_Na_formate
CHEBI_62965["CHEBI:62965"]
ing5_Na_formate -.-> CHEBI_62965
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002124 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002124 --> ing7_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing7_Nitrilotriacetic_acid -.-> CHEBI_44557
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002124 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_002124 --> ing9_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing9_MnSO4_x_H2O -.-> CHEBI_86364
ing10_NaCl["NaCl"]
CultureMech_002124 --> ing10_NaCl
CHEBI_26710["CHEBI:26710"]
ing10_NaCl -.-> CHEBI_26710
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002124 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002124 --> ing12_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing12_CoSO4_x_7_H2O -.-> CHEBI_91244
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002124 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002124 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002124 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002124 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_H3BO3["H3BO3"]
CultureMech_002124 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002124 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002124 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002124 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002124 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002124 --> ing22_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing22_SrCl2_x_6_H2O -.-> CHEBI_36385
ing23_KI["KI"]
CultureMech_002124 --> ing23_KI
CHEBI_8346["CHEBI:8346"]
ing23_KI -.-> CHEBI_8346
ing24_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002124 --> ing24_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing24_MgCl2_x_6_H2O -.-> CHEBI_86345
ing25_Na3_citrate["Na3-citrate"]
CultureMech_002124 --> ing25_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing25_Na3_citrate -.-> CHEBI_53258
ing26_KCl["KCl"]
CultureMech_002124 --> ing26_KCl
CHEBI_32588["CHEBI:32588"]
ing26_KCl -.-> CHEBI_32588
ing27_NaBr["NaBr"]
CultureMech_002124 --> ing27_NaBr
CHEBI_63004["CHEBI:63004"]
ing27_NaBr -.-> CHEBI_63004
ing28_Biotin["Biotin"]
CultureMech_002124 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_002124 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_002124 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.326409 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.326409 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Sulfur
S 32.067 g/mol
|
9.8912 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.48368 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
Na-formate
CHO2.Na 68.007 g/mol
|
2.96736 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.593472 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
17.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.0, 14.0] |
| 10 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 11 |
NaCl
Cl.Na 58.443 g/mol
|
56.4 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 1.0, 55.4] |
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.1, 1.5] |
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.06999999999999999 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.01, 0.06] |
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.03 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 24 |
KI
I.K 166.002 g/mol
|
0.0001 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 25 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
11 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 26 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
0.02 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 27 |
KCl
Cl.K 74.551 g/mol
|
1.3 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 28 |
NaBr
Br.Na 102.894 g/mol
|
0.2 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 35 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 36 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 37 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 38 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except sulfur, carbonate, formate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. For sterilization heat cultivation vessels for 1 - 2 hours to 90 - 100°C in a water bath on each of three successive days. Prior to inoculation add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and formate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are sterilized by filtration. Adjust pH of complete medium to 6.8, if necessary.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.858668Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 946 |
| 2026-02-06T05:10:47.235341Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:32.262144Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:10.543120Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002124 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.220613+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NAUTILIA MEDIUM" to "nautilia_medium" for consistent matching |
| 2026-06-05T07:41:39.452380+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.470692+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |