Overview
- External ontology
- mediadive.medium:950 (source link unavailable) — ONR7a MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium950.pdf
Composition
flowchart LR
CultureMech_002128["onr7a_medium"]
ing0_NaCl["NaCl"]
CultureMech_002128 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Na2SO4["Na2SO4"]
CultureMech_002128 --> ing1_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing1_Na2SO4 -.-> CHEBI_32149
ing2_KCl["KCl"]
CultureMech_002128 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_NaBr["NaBr"]
CultureMech_002128 --> ing3_NaBr
CHEBI_63004["CHEBI:63004"]
ing3_NaBr -.-> CHEBI_63004
ing4_NaHCO3["NaHCO3"]
CultureMech_002128 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_H3BO3["H3BO3"]
CultureMech_002128 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_NaF["NaF"]
CultureMech_002128 --> ing6_NaF
CHEBI_28741["CHEBI:28741"]
ing6_NaF -.-> CHEBI_28741
ing7_NH4Cl["NH4Cl"]
CultureMech_002128 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
CultureMech_002128 --> ing8_Na2HPO4_x_7_H2O
CHEBI_34683["CHEBI:34683"]
ing8_Na2HPO4_x_7_H2O -.-> CHEBI_34683
ing9_TAPSO["TAPSO"]
CultureMech_002128 --> ing9_TAPSO
CHEBI_84015["CHEBI:84015"]
ing9_TAPSO -.-> CHEBI_84015
ing10_Agar["Agar"]
CultureMech_002128 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
ing11_Agarose["Agarose"]
CultureMech_002128 --> ing11_Agarose
CHEBI_2511["CHEBI:2511"]
ing11_Agarose -.-> CHEBI_2511
ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002128 --> ing12_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002128 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002128 --> ing14_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing14_SrCl2_x_6_H2O -.-> CHEBI_36385
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002128 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
45.58 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Na2SO4
2Na.O4S 142.043 g/mol
|
7.96 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
1.44 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
NaBr
Br.Na 102.894 g/mol
|
0.166 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.062 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
0.054 G_PER_L |
CHEBI:33118
boric acid
|
|
| 7 |
NaF
F.Na 41.988 g/mol
|
0.0052 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.54 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Na2HPO4 x 7 H2O
HO4P.2Na 141.958 g/mol
|
0.178 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 10 |
TAPSO
C15H18Cl2N2O 313.228 g/mol
|
2.6 G_PER_L |
CHEBI:84015
(1R,2R)-diclocymet
|
|
| 11 | Agar | 30 G_PER_L |
CHEBI:2509
agar
|
(for solid medium, alternative) |
| 12 |
Agarose
(C12H18O9)n.C12H20O10 630.549 g/mol
|
24 G_PER_L |
CHEBI:2511
agarose
|
(for solid medium, alternative) |
| 13 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
24.8444 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.24444 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.0533333 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.04 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Preparation
- Autoclave the solutions separately. All solutions should be mixed after autoclaving when they have cooled to at least 50°C. For solid media add to the first solution 15 g/l of Bacto agar or 12 g/l of agarose (Sigma).
- adjust pH to 7.6 with NaOH
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.878031Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 950 |
| 2026-03-13T06:19:10.588402Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002128 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.273014+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ONR7a MEDIUM" to "onr7a_medium" for consistent matching |
| 2026-06-05T07:41:40.523485+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.774971+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |