methylophaga_sulfidovorans_medium

CultureMech:002129 bacterial DEFINED SOLID_AGAR
Original name: METHYLOPHAGA SULFIDOVORANS MEDIUM

Overview

External ontology
mediadive.medium:951 (source link unavailable) — METHYLOPHAGA SULFIDOVORANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.8}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium951.pdf

Composition

flowchart LR
    CultureMech_002129["methylophaga_sulfidovorans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002129 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002129 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_002129 --> ing2_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing3_KCl["KCl"]
    CultureMech_002129 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002129 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002129 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2CO3["Na2CO3"]
    CultureMech_002129 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002129 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Methanol["Methanol"]
    CultureMech_002129 --> ing8_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing8_Methanol -.-> CHEBI_17790
    ing9_Agar["Agar"]
    CultureMech_002129 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_HCl["HCl"]
    CultureMech_002129 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_ZnCl2["ZnCl2"]
    CultureMech_002129 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002129 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_002129 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002129 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002129 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002129 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002129 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_Biotin["Biotin"]
    CultureMech_002129 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Pantothenate["Pantothenate"]
    CultureMech_002129 --> ing19_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing19_Pantothenate -.-> CHEBI_16454
    ing20_Riboflavine["Riboflavine"]
    CultureMech_002129 --> ing20_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavine -.-> CHEBI_17015
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_002129 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
15 G_PER_L CHEBI:26710
sodium chloride
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
3 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.33 G_PER_L CHEBI:91243
calcium chloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.02 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2CO3
CO3.2Na 105.988 g/mol
2 G_PER_L CHEBI:29377
sodium carbonate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
10 Agar 15 G_PER_L CHEBI:2509
agar
11 HCl
HCl 36.461 g/mol
5 G_PER_L CHEBI:17883
hydrogen chloride
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.0696517 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.199005 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.00597015 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.189055 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.00199005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0238806 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0358209 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
20 Pantothenate
C9H16NO5 218.229 g/mol
0.05 G_PER_L CHEBI:16454
pantothenate
21 Riboflavine
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
22 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Prepare the medium without methanol and the vitamin solution. Adjust pH to 7.5 (±0.3) with 2 N HCl. Liquid media is prepared in either Hungate tubes (5ml medium) or 100ml serum bottles (10ml medium) sealed with rubber stoppers.
  2. Sterilize by autoclaving. After autoclaving add the methanol (filter sterilised) and the vitamin solution (filter sterilised) from sterile stock solutions.

Solutions

Trace element solution SL10

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.49254 G_PER_L

Vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Nicotinic acid 0.2 G_PER_L
Thiamine 0.1 G_PER_L
p-Aminobenzoic acid 0.1 G_PER_L
Pyridoxine hydrochloride 0.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.880697Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 951
2026-03-13T06:19:10.599060Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002129
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.285755+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOPHAGA SULFIDOVORANS MEDIUM" to "methylophaga_sulfidovorans_medium" for consistent matching
2026-05-20T06:01:56.933976+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.8' -> {'min': 7.2, 'max': 7.8}
2026-06-05T07:41:33.341299+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.640297+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.577962+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL10' @ 1 ml/l; 4 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).