Overview
- External ontology
- mediadive.medium:957 (source link unavailable) — MICROVIRGULA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium957.pdf
Composition
flowchart LR
CultureMech_002135["microvirgula_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002135 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002135 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002135 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002135 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Yeast_extract["Yeast extract"]
CultureMech_002135 --> ing4_Yeast_extract
ing5_Sodium_succinate["Sodium succinate"]
CultureMech_002135 --> ing5_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing5_Sodium_succinate -.-> CHEBI_63675
ing6_KNO3["KNO3"]
CultureMech_002135 --> ing6_KNO3
CHEBI_63043["CHEBI:63043"]
ing6_KNO3 -.-> CHEBI_63043
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.82 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.7 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 | Yeast extract | 0.25 G_PER_L | ||
| 6 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1.5 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 7 |
KNO3
K.NO3 101.102 g/mol
|
1.5 G_PER_L |
CHEBI:63043
potassium nitrate
|
Preparation
- Adjust pH 7.0, anaerobic with N2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.891561Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 957 |
| 2026-03-13T06:19:10.624792Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002135 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.317677+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MICROVIRGULA MEDIUM" to "microvirgula_medium" for consistent matching |
| 2026-06-05T07:41:33.859560+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.595140+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:07.823733+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |