Overview
- External ontology
- mediadive.medium:959a (source link unavailable) — MARINITOGA LITORALIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium959a.pdf
Composition
flowchart LR
CultureMech_002138["marinitoga_litoralis_medium"]
ing0_NaCl["NaCl"]
CultureMech_002138 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002138 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002138 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002138 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KCl["KCl"]
CultureMech_002138 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_PIPES["PIPES"]
CultureMech_002138 --> ing5_PIPES
CHEBI_39033["CHEBI:39033"]
ing5_PIPES -.-> CHEBI_39033
ing6_K2HPO4["K2HPO4"]
CultureMech_002138 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_KH2PO4["KH2PO4"]
CultureMech_002138 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
CultureMech_002138 --> ing8_Na_acetate_x_3_H2O
CHEBI_32138["CHEBI:32138"]
ing8_Na_acetate_x_3_H2O -.-> CHEBI_32138
ing9_Yeast_extract["Yeast extract"]
CultureMech_002138 --> ing9_Yeast_extract
ing10_Trypticase_peptone["Trypticase peptone"]
CultureMech_002138 --> ing10_Trypticase_peptone
ing11_Sodium_resazurin["Sodium resazurin"]
CultureMech_002138 --> ing11_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Sodium_resazurin -.-> CHEBI_8806
ing12_Maltose["Maltose"]
CultureMech_002138 --> ing12_Maltose
CHEBI_17306["CHEBI:17306"]
ing12_Maltose -.-> CHEBI_17306
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002138 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
26 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 | PIPES | 3.4 G_PER_L |
CHEBI:39033
PIPES
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.35 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.35 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
|
2.72 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 10 | Yeast extract | 2 G_PER_L | ||
| 11 | Trypticase peptone | 2 G_PER_L | ||
| 12 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
Maltose
C12H22O11 342.297 g/mol
|
3.5 G_PER_L |
CHEBI:17306
maltose
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 6.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.898500Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 959a |
| 2026-03-13T06:19:10.646579Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002138 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.342874+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MARINITOGA LITORALIS MEDIUM" to "marinitoga_litoralis_medium" for consistent matching |
| 2026-06-05T07:41:13.139380+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.969198+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_959_sl_ph_score10.py | RESOLVED_KOMODO_959_SL_PH_VARIANTS | DSMZ Medium 959a preserves SL medium components and concentrations but records pH 6 for Marinitoga litoralis. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |