Overview
- External ontology
- mediadive.medium:95 (source link unavailable) — DESULFUROMONAS ACETOXIDANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium95.pdf
Composition
flowchart LR
CultureMech_002139["desulfuromonas_acetoxidans_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002139 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_002139 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002139 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002139 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_NaCl["NaCl"]
CultureMech_002139 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002139 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Na2SO4["Na2SO4"]
CultureMech_002139 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Na2_DL_malate["Na2-DL-malate"]
CultureMech_002139 --> ing7_Na2_DL_malate
CHEBI_91260["CHEBI:91260"]
ing7_Na2_DL_malate -.-> CHEBI_91260
ing8_Yeast_extract["Yeast extract"]
CultureMech_002139 --> ing8_Yeast_extract
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_002139 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_Ethanol["Ethanol"]
CultureMech_002139 --> ing10_Ethanol
CHEBI_16236["CHEBI:16236"]
ing10_Ethanol -.-> CHEBI_16236
ing11_NaHCO3["NaHCO3"]
CultureMech_002139 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002139 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_002139 --> ing13_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing13_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002139 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_ZnCl2["ZnCl2"]
CultureMech_002139 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002139 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_H3BO3["H3BO3"]
CultureMech_002139 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002139 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002139 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002139 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002139 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.05152 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.315457 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.05152 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.10305 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
21.0305 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.105152 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.8612 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 | Na2-DL-malate | 2.79706 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 9 | Yeast extract | 0.525762 G_PER_L | ||
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000525762 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Ethanol
C2H6O 46.069 g/mol
|
0.3 G_PER_L |
CHEBI:16236
ethanol
|
|
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
46.25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Adjust pH of complete medium to 7.2, if necessary.
- Adjust pH to 6.0 with 2 N NaOH.
- Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.161071Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 95 |
| 2026-03-13T06:19:10.655676Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002139 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.353162+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFUROMONAS ACETOXIDANS MEDIUM" to "desulfuromonas_acetoxidans_medium" for consistent matching |
| 2026-06-05T07:40:58.390596+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.262759+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |