Overview
- External ontology
- mediadive.medium:964 (source link unavailable) — CAMINICELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium964.pdf
Composition
flowchart LR
CultureMech_002145["caminicella_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_002145 --> ing0_Sea_Salt
ing1_PIPES_buffer["PIPES buffer"]
CultureMech_002145 --> ing1_PIPES_buffer
ing2_D_Glucose["D-Glucose"]
CultureMech_002145 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_Sodium_resazurin["Sodium resazurin"]
CultureMech_002145 --> ing3_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Sodium_resazurin -.-> CHEBI_8806
ing4_Sulfur["Sulfur"]
CultureMech_002145 --> ing4_Sulfur
CHEBI_26833["CHEBI:26833"]
ing4_Sulfur -.-> CHEBI_26833
ing5_Peptone["Peptone"]
CultureMech_002145 --> ing5_Peptone
ing6_Yeast_extract["Yeast extract"]
CultureMech_002145 --> ing6_Yeast_extract
ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002145 --> ing7_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 30 G_PER_L | ||
| 2 | PIPES buffer | 6.05 G_PER_L | ||
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 4 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Sulfur
S 32.067 g/mol
|
12 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 6 | Peptone | 1 G_PER_L | ||
| 7 | Yeast extract | 0.5 G_PER_L | ||
| 8 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Dissolve ingredients (except sulfur, peptone, yeast extract, and sulfide), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 7.2 - 7.4, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and autoclave at <b>110°C</b> for 20 min. Add peptone, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.922393Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 964 |
| 2026-03-13T06:19:10.730588Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002145 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.439134+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CAMINICELLA MEDIUM" to "caminicella_medium" for consistent matching |
| 2026-06-05T07:40:52.461014+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |