Overview
- External ontology
- mediadive.medium:966 (source link unavailable) — R3 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium966.pdf
Composition
flowchart LR
CultureMech_002147["r3_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002147 --> ing0_Yeast_extract
ing1_Proteose_peptone["Proteose peptone"]
CultureMech_002147 --> ing1_Proteose_peptone
ing2_Casamino_acids["Casamino acids"]
CultureMech_002147 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_002147 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_K2HPO4["K2HPO4"]
CultureMech_002147 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002147 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Na_pyruvate["Na pyruvate"]
CultureMech_002147 --> ing6_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_Na_pyruvate -.-> CHEBI_50144
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Proteose peptone | 1 G_PER_L | ||
| 3 | Casamino acids | 1 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
Na pyruvate
C3H3O3.Na 110.044 g/mol
|
0.05 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Preparation
- Final pH should be 7.8
- Autoclave at 121°C for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.928319Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 966 |
| 2026-01-28T19:48:35.257662Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:10.747583Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002147 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.458103+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R3 MEDIUM" to "r3_medium" for consistent matching |
| 2026-06-05T07:41:43.703742+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.063637+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:10.811465+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |