Overview
- External ontology
- mediadive.medium:969 (source link unavailable) — SULFOBACILLUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 1.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium969.pdf
Composition
flowchart LR
CultureMech_002151["sulfobacillus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002151 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_002151 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002151 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002151 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_002151 --> ing4_Yeast_extract
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002151 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Na_tetrathionate["Na-tetrathionate"]
CultureMech_002151 --> ing6_Na_tetrathionate
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 0.2 G_PER_L | ||
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
14 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 | Na-tetrathionate | 0.1 G_PER_L |
Preparation
- pH should be 1.8.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.940654Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 969 |
| 2026-03-13T06:19:10.783977Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002151 |
| 2026-03-17T06:30:29.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.500118+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SULFOBACILLUS MEDIUM" to "sulfobacillus_medium" for consistent matching |
| 2026-06-05T07:40:16.364558+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.683212+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |