myct_medium (CultureMech:002154)

Overview

External ontology

mediadive.medium:972 (source link unavailable) — MYCT MEDIUM

Applications

Microbial cultivation

pH

6.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium972.pdf

Composition

flowchart LR
    CultureMech_002154["myct_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002154 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002154 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgCl2["MgCl2"]
    CultureMech_002154 --> ing2_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing2_MgCl2 -.-> CHEBI_6636
    ing3_Sodium_citrate["Sodium citrate"]
    CultureMech_002154 --> ing3_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing3_Sodium_citrate -.-> CHEBI_53258
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002154 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_002154 --> ing5_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing5_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002154 --> ing6_Yeast_extract
    ing7_Casein_hydrolysate["Casein hydrolysate"]
    CultureMech_002154 --> ing7_Casein_hydrolysate
    ing8_Tween_80["Tween 80"]
    CultureMech_002154 --> ing8_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing8_Tween_80 -.-> CHEBI_53426
    ing9_Cyclomaltoheptaose["Cyclomaltoheptaose"]
    CultureMech_002154 --> ing9_Cyclomaltoheptaose
    CHEBI_495055["CHEBI:495055"]
    ing9_Cyclomaltoheptaose -.-> CHEBI_495055

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	KH2PO4 H2O4P.K 136.084 g/mol	13.6 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
2	(NH4)2SO4 2H4N.O4S 132.141 g/mol	4 G_PER_L	CHEBI:62946 ammonium sulfate
3	MgCl2 2Cl.Mg 95.211 g/mol	0.2 G_PER_L	CHEBI:6636 magnesium dichloride
4	Sodium citrate C6H5O7.3Na 258.069 g/mol	0.25 G_PER_L	CHEBI:53258 sodium citrate
5	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.025 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
6	MnSO4 x 4 H2O 4H2O.Mn.O4S 223.061 g/mol	0.025 G_PER_L	CHEBI:86358 manganese(II) sulfate tetrahydrate
7	Yeast extract	3 G_PER_L
8	Casein hydrolysate	3 G_PER_L
9	Tween 80 (C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol	1 G_PER_L	CHEBI:53426 polysorbate 80
10	Cyclomaltoheptaose C42H70O35 1134.987 g/mol	7 G_PER_L	CHEBI:495055 beta-cyclodextrin

Preparation

pH 6.5.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:12.945385Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 972
2026-03-13T06:19:10.797028Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002154
2026-03-17T06:30:55.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.515820+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MYCT MEDIUM" to "myct_medium" for consistent matching
2026-06-05T07:41:39.057509+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.339338+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.