methylophaga_alcalica_medium

CultureMech:002158 bacterial DEFINED SOLID_AGAR
Original name: METHYLOPHAGA ALCALICA MEDIUM

Overview

External ontology
mediadive.medium:976 (source link unavailable) — METHYLOPHAGA ALCALICA MEDIUM
Applications
  • Microbial cultivation
pH
9.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium976.pdf

Composition

flowchart LR
    CultureMech_002158["methylophaga_alcalica_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002158 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_KNO3["KNO3"]
    CultureMech_002158 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002158 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaCl["NaCl"]
    CultureMech_002158 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002158 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_Methanol["Methanol"]
    CultureMech_002158 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790
    ing6_Ferric_citrate["Ferric citrate"]
    CultureMech_002158 --> ing6_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing6_Ferric_citrate -.-> CHEBI_144421
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002158 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_MgCl2_x_4_H2O["MgCl2 x 4 H2O"]
    CultureMech_002158 --> ing8_MgCl2_x_4_H2O
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002158 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002158 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_Agar["Agar"]
    CultureMech_002158 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.22 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
5 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
6 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
7 Ferric citrate
C6H5FeO7 244.944 g/mol
0.03 G_PER_L CHEBI:144421
iron(III) citrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.03 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 MgCl2 x 4 H2O 0.005 G_PER_L
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.005 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.5 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
12 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)

Preparation

  1. Final pH 9.5
  2. Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:12.957291Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 976
2026-03-13T06:19:10.831864Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002158
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.554237+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOPHAGA ALCALICA MEDIUM" to "methylophaga_alcalica_medium" for consistent matching
2026-06-05T07:41:33.272288+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.561109+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.615046+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.