halobacterium_medium (CultureMech:002162)

Overview

External ontology

mediadive.medium:97 (source link unavailable) — HALOBACTERIUM MEDIUM

Applications

Microbial cultivation

pH

7.4

High-metal flag

True

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium97.pdf

Composition

flowchart LR
    CultureMech_002162["halobacterium_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_002162 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002162 --> ing1_Yeast_extract
    ing2_Na3_citrate["Na3-citrate"]
    CultureMech_002162 --> ing2_Na3_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Na3_citrate -.-> CHEBI_53258
    ing3_KCl["KCl"]
    CultureMech_002162 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002162 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002162 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_002162 --> ing6_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing6_MnSO4_x_H2O -.-> CHEBI_86364
    ing7_NaCl["NaCl"]
    CultureMech_002162 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar["Agar"]
    CultureMech_002162 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

#	Ingredient	Concentration	Ontology
1	Casamino acids	7.5 G_PER_L
2	Yeast extract	10 G_PER_L
3	Na3-citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
4	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	20 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.05 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
7	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.0002 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
8	NaCl Cl.Na 58.443 g/mol	250 G_PER_L	CHEBI:26710 sodium chloride
9	Agar	20 G_PER_L	CHEBI:2509 agar

Preparation

Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:10.164247Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 97
2026-01-28T19:48:21.641319Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:10.881416Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002162
2026-03-17T06:30:11.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.607235+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "HALOBACTERIUM MEDIUM" to "halobacterium_medium" for consistent matching
2026-06-05T07:41:07.603225+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:39.670905+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-07T10:55:41.454416+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.