Overview
- External ontology
- mediadive.medium:984 (source link unavailable) — NL 333-AGAR MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium984.pdf
Composition
flowchart LR
CultureMech_002166["nl_333_agar_medium"]
ing0_Glucose["Glucose"]
CultureMech_002166 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Starch["Starch"]
CultureMech_002166 --> ing1_Starch
CHEBI_28017["CHEBI:28017"]
ing1_Starch -.-> CHEBI_28017
ing2_Malt_extract["Malt extract"]
CultureMech_002166 --> ing2_Malt_extract
ing3_Yeast_extract["Yeast extract"]
CultureMech_002166 --> ing3_Yeast_extract
ing4_Casein_peptone["Casein peptone"]
CultureMech_002166 --> ing4_Casein_peptone
ing5_Ammonium_nitrate["Ammonium nitrate"]
CultureMech_002166 --> ing5_Ammonium_nitrate
CHEBI_63038["CHEBI:63038"]
ing5_Ammonium_nitrate -.-> CHEBI_63038
ing6_CaCO3["CaCO3"]
CultureMech_002166 --> ing6_CaCO3
CHEBI_3311["CHEBI:3311"]
ing6_CaCO3 -.-> CHEBI_3311
ing7_Agar["Agar"]
CultureMech_002166 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Starch | 10 G_PER_L |
CHEBI:28017
starch
|
|
| 3 | Malt extract | 10 G_PER_L | ||
| 4 | Yeast extract | 3 G_PER_L | ||
| 5 | Casein peptone | 3 G_PER_L | ||
| 6 |
Ammonium nitrate
H4N.NO3 80.043 g/mol
|
3 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 7 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- For autoclaving the pH of the medium should be adjusted to 7.2.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:12.990468Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 984 |
| 2026-03-13T06:19:10.912427Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002166 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.641452+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NL 333-AGAR MEDIUM" to "nl_333_agar_medium" for consistent matching |
| 2026-06-05T07:41:39.941216+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |