Overview
- External ontology
- mediadive.medium:98 (source link unavailable) — RHIZOBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium98.pdf
Composition
flowchart LR
CultureMech_002171["rhizobium_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002171 --> ing0_Yeast_extract
ing1_Mannitol["Mannitol"]
CultureMech_002171 --> ing1_Mannitol
CHEBI_29864["CHEBI:29864"]
ing1_Mannitol -.-> CHEBI_29864
ing2_Agar["Agar"]
CultureMech_002171 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Distilled_water["Distilled water"]
CultureMech_002171 --> ing3_Distilled_water
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 |
Mannitol
C6H14O6 182.172 g/mol
|
10 G_PER_L |
CHEBI:29864
mannitol
|
|
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 4 | Distilled water | 0.8 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Preparation
- Adjust pH to 7.0.
Solutions
Soil extract
Dose: 0.2 L
| Ingredient | Concentration | Notes |
|---|---|---|
| air-dried garden soil | 400 G_PER_L | |
| Na2CO3 | 1 G_PER_L | |
| Distilled water | 0.2 L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.165786Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 98 |
| 2026-03-13T06:19:10.947511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002171 |
| 2026-03-17T06:30:26.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.681015+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHIZOBIUM MEDIUM" to "rhizobium_medium" for consistent matching |
| 2026-06-05T07:41:44.208638+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.909146+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T11:35:03.558874+00:00 | codex | RESTORED_REVIEWED_MEDIUM_STOCK_BOUNDARIES | Exact source graph, complete ingredient inventory, amounts, and original PDF recipe chain checked. Numeric concentrations and chemical mappings preserved; stock doses and water are explicit batch amounts. No final-medium stock dilution inferred. Parent/variant relationships remain separately unreviewed. |