Overview
- External ontology
- mediadive.medium:991 (source link unavailable) — CALDISPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 4.0, 'max': 4.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium991.pdf
Composition
flowchart LR
CultureMech_002173["caldisphaera_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002173 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_002173 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002173 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002173 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002173 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002173 --> ing5_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_002173 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_Sulfur["Sulfur"]
CultureMech_002173 --> ing7_Sulfur
CHEBI_26833["CHEBI:26833"]
ing7_Sulfur -.-> CHEBI_26833
ing8_Yeast_extract["Yeast extract"]
CultureMech_002173 --> ing8_Yeast_extract
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002173 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002173 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002173 --> ing11_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002173 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002173 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002173 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_VOSO4_x_2_H2O["VOSO4 x 2 H2O"]
CultureMech_002173 --> ing15_VOSO4_x_2_H2O
CHEBI_87009["CHEBI:87009"]
ing15_VOSO4_x_2_H2O -.-> CHEBI_87009
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002173 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_Biotin["Biotin"]
CultureMech_002173 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_002173 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002173 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_002173 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_Riboflavin["Riboflavin"]
CultureMech_002173 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_002173 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_002173 --> ing23_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_002173 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002173 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_002173 --> ing26__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26__DL__alpha_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.28586 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.276954 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.24728 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0692384 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.0197824 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.90801 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.00049456 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Sulfur
S 32.067 g/mol
|
9.8912 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 9 | Yeast extract | 0.49456 G_PER_L | ||
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49456 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.18 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.45 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.005 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
VOSO4 x 2 H2O
2H2O.O4S.OV 199.036 g/mol
|
0.003 G_PER_L |
CHEBI:87009
vanadyl sulfate dihydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.001 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except sulfur, yeast extract, vitamins and sulfide), adjust pH to 3.5 with 10 N H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 100 ml bottles) containing already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a water bath to 90 - 100°C for 1 - 2 hours on each of 3 successive days. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 4.0 - 4.5, if necessary.
- After inoculation pressurize vials to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- Adjust pH of final solution to 2 with 1 N HCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.006717Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 991 |
| 2026-01-28T19:48:11.995226Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:18.326866Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:10.963018Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002173 |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:23.697981+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CALDISPHAERA MEDIUM" to "caldisphaera_medium" for consistent matching |
| 2026-05-20T06:01:10.739452+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '4-4.5' -> {'min': 4.0, 'max': 4.5} |
| 2026-06-05T07:40:52.239217+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.201526+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |