bacillus_filiformis_medium (CultureMech:002174)

Overview

External ontology

mediadive.medium:992 (source link unavailable) — BACILLUS FILIFORMIS MEDIUM

Applications

Microbial cultivation

pH

9.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium992.pdf

Composition

flowchart LR
    CultureMech_002174["bacillus_filiformis_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002174 --> ing0_Yeast_extract
    ing1_Sodium_citrate["Sodium citrate"]
    CultureMech_002174 --> ing1_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing1_Sodium_citrate -.-> CHEBI_53258
    ing2_KCl["KCl"]
    CultureMech_002174 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002174 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002174 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_NaCl["NaCl"]
    CultureMech_002174 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002174 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002174 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	Yeast extract	10 G_PER_L
2	Sodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
3	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
5	Na2CO3 CO3.2Na 105.988 g/mol	3 G_PER_L	CHEBI:29377 sodium carbonate
6	NaCl Cl.Na 58.443 g/mol	100 G_PER_L	CHEBI:26710 sodium chloride
7	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
8	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	50 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate

Preparation

Sodium carbonate and sodium chloride should separately be autoclaved. pH 9.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.010820Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 992
2026-03-13T06:19:10.970282Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002174
2026-03-17T06:29:41.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.706465+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BACILLUS FILIFORMIS MEDIUM" to "bacillus_filiformis_medium" for consistent matching
2026-06-05T07:40:50.245770+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.707009+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.