isp_5_medium

CultureMech:002175 bacterial DEFINED SOLID_AGAR
Original name: ISP 5 MEDIUM

Overview

External ontology
mediadive.medium:993 (source link unavailable) — ISP 5 MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993.pdf

Composition

flowchart LR
    CultureMech_002175["isp_5_medium"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_002175 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Glycerol["Glycerol"]
    CultureMech_002175 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002175 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar["Agar"]
    CultureMech_002175 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
2 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
  2. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.012522Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 993
2026-03-13T06:19:10.974837Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002175
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.711712+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ISP 5 MEDIUM" to "isp_5_medium" for consistent matching
2026-05-20T06:01:23.374367+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-7.4' -> {'min': 7.0, 'max': 7.4}
2026-06-05T07:41:09.687617+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.151739+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.378662+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).