nesterenkonia_modified_medium

CultureMech:002176 bacterial COMPLEX SOLID_AGAR
Original name: NESTERENKONIA MODIFIED MEDIUM

Overview

External ontology
mediadive.medium:993a (source link unavailable) — NESTERENKONIA MODIFIED MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium993a.pdf

Composition

flowchart LR
    CultureMech_002176["nesterenkonia_modified_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002176 --> ing0_Yeast_extract
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002176 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_L_Asparagine["L-Asparagine"]
    CultureMech_002176 --> ing2_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing2_L_Asparagine -.-> CHEBI_17196
    ing3_Glycerol["Glycerol"]
    CultureMech_002176 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_K2HPO4["K2HPO4"]
    CultureMech_002176 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_002176 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
100 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
4 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
5 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Agar 20 G_PER_L CHEBI:2509
agar

Preparation

  1. Use medium 993 supplemented with Yeast extract (0.5%) and MgCl2 x 6 H20 (10%).
  2. The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
  3. Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.014403Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 993a
2026-03-13T06:19:10.979940Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002176
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.717502+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NESTERENKONIA MODIFIED MEDIUM" to "nesterenkonia_modified_medium" for consistent matching
2026-06-05T07:41:39.593909+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.523375+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.736078+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).