halorhodospira_medium

CultureMech:002184 bacterial DEFINED LIQUID
Original name: HALORHODOSPIRA MEDIUM

Overview

External ontology
mediadive.medium:999 (source link unavailable) — HALORHODOSPIRA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium999.pdf

Composition

flowchart LR
    CultureMech_002184["halorhodospira_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002184 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002184 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002184 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002184 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002184 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002184 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002184 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002184 --> ing7_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing7_Na2S_x_9_H2O -.-> CHEBI_76209
    ing8_H3BO3["H3BO3"]
    CultureMech_002184 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002184 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002184 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
    CultureMech_002184 --> ing11_MnCl2_x_2_H2O
    CHEBI_131395["CHEBI:131395"]
    ing11_MnCl2_x_2_H2O -.-> CHEBI_131395
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002184 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002184 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002184 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002184 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_Biotine["Biotine"]
    CultureMech_002184 --> ing16_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotine -.-> CHEBI_15956
    ing17_p_Aminobenzoate["p-Aminobenzoate"]
    CultureMech_002184 --> ing17_p_Aminobenzoate
    CHEBI_17836["CHEBI:17836"]
    ing17_p_Aminobenzoate -.-> CHEBI_17836
    ing18_Thiamine["Thiamine"]
    CultureMech_002184 --> ing18_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing18_Thiamine -.-> CHEBI_18385
    ing19_Pantothenate["Pantothenate"]
    CultureMech_002184 --> ing19_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing19_Pantothenate -.-> CHEBI_16454
    ing20_Pyridoxamine["Pyridoxamine"]
    CultureMech_002184 --> ing20_Pyridoxamine
    CHEBI_16410["CHEBI:16410"]
    ing20_Pyridoxamine -.-> CHEBI_16410
    ing21_Vitamine_B12["Vitamine B12"]
    CultureMech_002184 --> ing21_Vitamine_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamine_B12 -.-> CHEBI_176843
    ing22_Nicotinate["Nicotinate"]
    CultureMech_002184 --> ing22_Nicotinate
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinate -.-> CHEBI_15940

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
100 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.75 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
9 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
0.05 G_PER_L CHEBI:131395
manganese(II) chloride dihydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 Biotine
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
18 p-Aminobenzoate
C7H6NO2 136.13 g/mol
0.01 G_PER_L CHEBI:17836
4-aminobenzoate
19 Thiamine
C12H17N4OS 265.362 g/mol
0.01 G_PER_L CHEBI:18385
thiamine(1+)
20 Pantothenate
C9H16NO5 218.229 g/mol
0.005 G_PER_L CHEBI:16454
pantothenate
21 Pyridoxamine
C8H12N2O2 168.196 g/mol
0.05 G_PER_L CHEBI:16410
pyridoxamine
22 Vitamine B12 0.02 G_PER_L CHEBI:176843
vitamin B12
23 Nicotinate
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid

Preparation

  1. After autoclaving 20 min at 121°C, the following sterile solutions are added:
  2. The pH is adjusted to pH 7.2-7.4 For better growth of pure cultures, the growth medium should be supplemented with acetate (2 mM final concentration), succinate (1 mM final concentration) and yeast extract (0.5 g/l). Pure cultures should be incubated at 30°C and light intensity of 500 lux from a tungsten lamp (16 h light, 8 h dark). Pure cultures in liquid medium can be stored in 60 ml screw cap bottles at 4°C in the dark.
  3. Sterilized by filtration.
  4. Sterilized by filtration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.038425Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 999
2026-02-06T05:10:29.163762Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:11.053552Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002184
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:23.797982+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALORHODOSPIRA MEDIUM" to "halorhodospira_medium" for consistent matching
2026-05-20T06:01:22.310412+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4}
2026-06-05T07:41:08.138536+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.638924+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.