acidic_hb_1_medium

Overview

External ontology

mediadive.medium:J1003 (source link unavailable) — ACIDIC HB-1 MEDIUM

Applications

• Microbial cultivation

pH

5.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1003

Composition

flowchart LR
    CultureMech_002189["acidic_hb_1_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_002189 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MES["MES"]
    CultureMech_002189 --> ing1_MES
    CHEBI_39010["CHEBI:39010"]
    ing1_MES -.-> CHEBI_39010
    ing2_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002189 --> ing2_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing2_Na2S_x_9_H2O -.-> CHEBI_76209
    ing3_NaCl["NaCl"]
    CultureMech_002189 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002189 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002189 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_KCl["KCl"]
    CultureMech_002189 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaBr["NaBr"]
    CultureMech_002189 --> ing7_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing7_NaBr -.-> CHEBI_63004
    ing8_H3BO3["H3BO3"]
    CultureMech_002189 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002189 --> ing9_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing9_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing10__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002189 --> ing10__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing10__NH4_2SO4 -.-> CHEBI_62946
    ing11_KI["KI"]
    CultureMech_002189 --> ing11_KI
    CHEBI_8346["CHEBI:8346"]
    ing11_KI -.-> CHEBI_8346
    ing12_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
    CultureMech_002189 --> ing12_Na2WO2_x_2_H2O
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002189 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_KH2PO4["KH2PO4"]
    CultureMech_002189 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002189 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Trace_minerals__see_Medium_No__151["Trace minerals (see Medium No. 151"]
    CultureMech_002189 --> ing16_Trace_minerals__see_Medium_No__151
    ing17_Resazurin["Resazurin"]
    CultureMech_002189 --> ing17_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing17_Resazurin -.-> CHEBI_8806
    ing18_PIPES["PIPES"]
    CultureMech_002189 --> ing18_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing18_PIPES -.-> CHEBI_39033

Ingredients (19)

Preparation

1. Use Medium No. 744. Before autoclaving, adjust pH of the medium to 5.5. After autoclaving, add per liter the following solutions aseptically and anaerobically from anaerobic stocks:
2. Otherwise, prepare the medium as described in the recipe of Medium No. 744.
3. Mix ingredients, bring to a boil and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 10 ml 5% Na2S x 9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 7.0 with NaOH. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Before inoculation, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
4. Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance